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Literature summary for 1.1.1.214 extracted from
- Ketopantoic acid and ketopantoyl lactone reductases. Stereospecificity of transfer of hydrogen from reduced nicotinamide adenine dinucleotide phosphate (1975), J. Biol. Chem., 250, 2311-2314.
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Escherichia coli | the physiological substrate for the enzyme is uncertain, the physiological function of the enzyme is unknown | ? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| form A and form B of the enzyme obtained separated by rupture, centrifugation and column chromatography on DEAE-cellulose and hydroxylapatite | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| keto-omega-methylpantoyl lactone + NADPH | the only compound other than ketopantoyl lactone which is a substrate, 73% relative activity to ketopantoyl lactone with form A enzyme, 56% relative activity to ketopantoyl lactone with form B enzyme | Escherichia coli | methylpantoyl lactone + NADP+ | - |
? | |
| ketopantoyl lactone + NADPH | - |
Escherichia coli | pantoyl lactone + NADP+ | - |
? | |
| additional information | both forms of the enzyme are B-specific, the enzyme exhibits opposite stereospecificity from that observed with the Saccharomyces cerevisiae enzyme, which is A-specific, less than 5% relative activity to ketopantoyl lactone with the following 2-keto-gamma-lactones: 2-keto-4-hydroxy-3-methylbutyric acid-gamma-lactone, 2-keto-4-hydroxybutyric acid-gamma-lactone | Escherichia coli | ? | - |
? | |
| additional information | the physiological substrate for the enzyme is uncertain, the physiological function of the enzyme is unknown | Escherichia coli | ? | - |
? | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no activity observed with NADH | Escherichia coli | |
| NADPH | - |
Escherichia coli | |
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Release 2023.1 (January 2023)
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