Cloned (Comment) | Organism |
---|---|
overexpression of wild-type and mutant enzymes in Escherichia coli C41(DE3) | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
F118A | site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme, effects of mutation on binding constants and kinetics, overview | Rattus norvegicus |
F129A | site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme, effects of mutation on binding constants and kinetics, overview | Rattus norvegicus |
L54A | site-directed mutagenesis, reduced activity compared to the wild-type enzyme, effects of mutation on binding constants and kinetics, overview | Rattus norvegicus |
N306A | site-directed mutagenesis, reduced activity compared to the wild-type enzyme, effects of mutation on binding constants and kinetics, overview | Rattus norvegicus |
T226A | site-directed mutagenesis, reduced activity compared to the wild-type enzyme, effects of mutation on binding constants and kinetics, overview | Rattus norvegicus |
T24A | site-directed mutagenesis, reduced activity compared to the wild-type enzyme, effects of mutation on binding constants and kinetics, overview | Rattus norvegicus |
W227A | site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme, 23fold decreased affinity for progesterone, effects of mutation on binding constants and kinetics, overview | Rattus norvegicus |
Y310A | site-directed mutagenesis, reduced activity compared to the wild-type enzyme, effects of mutation on binding constants and kinetics, overview | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
progesterone | competitive to testosterone | Rattus norvegicus | |
testosterone | competitive to progesterone | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant enzymes from Escherichia coli C41(DE3), to homogeneity | Rattus norvegicus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a 3alpha-hydroxysteroid + NAD(P)+ = a 3-oxosteroid + NAD(P)H + H+ | ordered bi bi reaction mechanism | Rattus norvegicus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.006 | - |
purified recombinant mutant W227A, substrate androsterone | Rattus norvegicus |
0.01 | - |
purified recombinant mutant F118A, substrate androsterone | Rattus norvegicus |
0.03 | - |
purified recombinant mutant F129A, substrate androsterone | Rattus norvegicus |
0.08 | - |
purified recombinant mutant L54A, substrate androsterone | Rattus norvegicus |
0.09 | - |
purified recombinant mutant N306A, substrate androsterone | Rattus norvegicus |
0.1 | - |
purified recombinant mutant Y310A, substrate androsterone | Rattus norvegicus |
0.86 | - |
purified recombinant mutant T24A, substrate androsterone | Rattus norvegicus |
0.96 | - |
purified recombinant mutant T226A, substrate androsterone | Rattus norvegicus |
1.6 | - |
purified recombinant wild-type enzyme, substrate androsterone | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5beta-androstan-3alpha-ol-17-one + NAD(P)+ | - |
Rattus norvegicus | 5beta-androstane-3,17-dione + NAD(P)H | - |
r | |
5beta-pregnan-3alpha-ol-20-one + NAD(P)+ | - |
Rattus norvegicus | 5beta-pregnane-3,20-dione + NAD(P)H | - |
r | |
androsterone + NAD(P)+ | - |
Rattus norvegicus | 5alpha-androstane-3,17-dione + NAD(P)H | - |
r | |
progesterone + NAD(P)H | - |
Rattus norvegicus | 3alpha-hydroxy-pregn-4-ene-20-one + NAD(P)+ | - |
r | |
testosterone + NAD(P)H + H+ | - |
Rattus norvegicus | 4-androsten-3alpha,17beta-diol + NAD(P)+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
3alpha-HSD | - |
Rattus norvegicus |
3alpha-hydroxysteroid dehydrogenase | - |
Rattus norvegicus |
AKR1C9 | - |
Rattus norvegicus |
More | see also EC 1.1.1.50, 3alpha-hydroxysteroid dehydrogenase (B-specific), for enzymes catalyzing the same reaction, but which are B-specific with respect to hydrogen transfer to/from NAD(P)+/NAD(P)H. Enzymes without information of stereospecificity are summarized under EC 1.1.1.50, but may in fact belong to EC 1.1.1.213 | Rattus norvegicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kcat in steady-state, single turnover, burst in multiple turnover, steady-state in multiple turnover | Rattus norvegicus | |
0.0053 | - |
androsterone | steady-state, pH 8.0, 25°C, recombinant mutant W227A | Rattus norvegicus | |
0.0083 | - |
androsterone | steady-state, pH 8.0, 25°C, recombinant mutant F118A | Rattus norvegicus | |
0.57 | - |
androsterone | steady-state, pH 8.0, 25°C, recombinant mutant T226A | Rattus norvegicus | |
0.83 | - |
androsterone | steady-state, pH 8.0, 25°C, recombinant wild-type enzyme | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD(P)+ | A-specific | Rattus norvegicus | |
NAD(P)H | A-specific | Rattus norvegicus |