Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | bile acid-incucible baiA gene family | Eubacterium sp. |
Cloned (Comment) | Organism |
---|---|
overexpressed in Escherichia coli HMS174(DE3) | Eubacterium sp. |
Protein Variants | Comment | Organism |
---|---|---|
additional information | potential CoA binding sites spanning residues 182 to 193. N-terminal region is thought to contain the NAD(P)+ binding site, the middle and C-terminal regions the active site of the enzyme | Eubacterium sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27000 | - |
PAGE, western blot | Eubacterium sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Eubacterium sp. | - |
- |
- |
Eubacterium sp. VPI 12708 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Centriprep-10 concentration, DEAE-HPLC | Eubacterium sp. |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a 3alpha-hydroxysteroid + NAD(P)+ = a 3-oxosteroid + NAD(P)H + H+ | short-chain alcohol/polyol dehydrogenase family | Eubacterium sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholic acid + NAD(P)+ | - |
Eubacterium sp. | 3-oxocholate + NAD(P)H | very little activity | ? | |
cholic acid + NAD(P)+ | - |
Eubacterium sp. VPI 12708 | 3-oxocholate + NAD(P)H | very little activity | ? | |
choloyl-CoA + NAD(P)+ | - |
Eubacterium sp. | 3-oxocholoyl-CoA + NAD(P)H + H+ | - |
r | |
choloyl-CoA + NAD(P)+ | - |
Eubacterium sp. VPI 12708 | 3-oxocholoyl-CoA + NAD(P)H + H+ | - |
r | |
deoxycholoyl-CoA + NAD(P)+ | 75% activity compared with cholyl-CoA | Eubacterium sp. | 3-oxodeoxycholoyl-CoA + NAD(P)H + H+ | - |
? | |
deoxycholoyl-CoA + NAD(P)+ | 75% activity compared with cholyl-CoA | Eubacterium sp. VPI 12708 | 3-oxodeoxycholoyl-CoA + NAD(P)H + H+ | - |
? | |
additional information | no activity with cholate, chenodeoxycholate, deoxycholate, glycocholate, taurocholate, ursodeoxycholate and bile acid | Eubacterium sp. | ? | - |
? | |
additional information | no activity with cholate, chenodeoxycholate, deoxycholate, glycocholate, taurocholate, ursodeoxycholate and bile acid | Eubacterium sp. VPI 12708 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | - |
Eubacterium sp. |