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Literature summary for 1.1.1.213 extracted from

  • Mallonee, D.H.; Lijewski, M.A.; Hylemon, P.B.
    Expression in Escherichia coli and characterization of a bile acid-inducible 3alpha-hydroxysteroid dehydrogenase from Eubacterium sp. strain VPI 12708 (1995), Curr. Microbiol., 30, 259-263.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information bile acid-incucible baiA gene family Eubacterium sp.

Cloned(Commentary)

Cloned (Comment) Organism
overexpressed in Escherichia coli HMS174(DE3) Eubacterium sp.

Protein Variants

Protein Variants Comment Organism
additional information potential CoA binding sites spanning residues 182 to 193. N-terminal region is thought to contain the NAD(P)+ binding site, the middle and C-terminal regions the active site of the enzyme Eubacterium sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
PAGE, western blot Eubacterium sp.

Organism

Organism UniProt Comment Textmining
Eubacterium sp.
-
-
-
Eubacterium sp. VPI 12708
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Centriprep-10 concentration, DEAE-HPLC Eubacterium sp.

Reaction

Reaction Comment Organism Reaction ID
a 3alpha-hydroxysteroid + NAD(P)+ = a 3-oxosteroid + NAD(P)H + H+ short-chain alcohol/polyol dehydrogenase family Eubacterium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cholic acid + NAD(P)+
-
Eubacterium sp. 3-oxocholate + NAD(P)H very little activity ?
cholic acid + NAD(P)+
-
Eubacterium sp. VPI 12708 3-oxocholate + NAD(P)H very little activity ?
choloyl-CoA + NAD(P)+
-
Eubacterium sp. 3-oxocholoyl-CoA + NAD(P)H + H+
-
r
choloyl-CoA + NAD(P)+
-
Eubacterium sp. VPI 12708 3-oxocholoyl-CoA + NAD(P)H + H+
-
r
deoxycholoyl-CoA + NAD(P)+ 75% activity compared with cholyl-CoA Eubacterium sp. 3-oxodeoxycholoyl-CoA + NAD(P)H + H+
-
?
deoxycholoyl-CoA + NAD(P)+ 75% activity compared with cholyl-CoA Eubacterium sp. VPI 12708 3-oxodeoxycholoyl-CoA + NAD(P)H + H+
-
?
additional information no activity with cholate, chenodeoxycholate, deoxycholate, glycocholate, taurocholate, ursodeoxycholate and bile acid Eubacterium sp. ?
-
?
additional information no activity with cholate, chenodeoxycholate, deoxycholate, glycocholate, taurocholate, ursodeoxycholate and bile acid Eubacterium sp. VPI 12708 ?
-
?

Subunits

Subunits Comment Organism
tetramer
-
Eubacterium sp.