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Literature summary for 1.1.1.213 extracted from

  • Tomkins, G.M.
    A mammalian 3alpha-hydroxysteroid dehydrogenase (1956), J. Biol. Chem., 218, 437-447.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 100% inhibition at 0.1 mM Rattus norvegicus
iodoacetate 50% inhibition at 0.1 mM Rattus norvegicus
p-chloromercuribenzoate non-competitive with respect to dihydrocortisone 46-100% inhibition at 0.01 mM, preincubation with NADH lowers the inhibitory effect Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
5beta-pregnane-17alpha,20beta,21-triol-3,11-dione
-
Rattus norvegicus
0.015
-
NADH
-
Rattus norvegicus
0.04
-
NAD+
-
Rattus norvegicus
0.04
-
NADP+
-
Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, Ca2(PO4)2 gel extraction, ethanol precipitation Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver also present in kidney and testis Rattus norvegicus
-
additional information low activities found in dog and rabbit liver, no significant activities are detected in extracts of beef, hog, guinea pig, mouse and human liver Rattus norvegicus
-

Storage Stability

Storage Stability Organism
-10°C, 3 weeks, activity declines about 50% Rattus norvegicus
-10°C, most purified preparation, 5 days, loses between 80% and 100% of activity Rattus norvegicus
3°C, first ammonium sulfate fraction, stable at least 3 weeks Rattus norvegicus
3°C, most purified preparation, 5 days, loses 50% of activity Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5beta-pregnane-17alpha,20beta,21-triol-3,11-dione + NAD(P)H
-
Rattus norvegicus 5beta-pregnane-3alpha,17alpha,20beta,21-tetraol-11-one + NAD(P)+
-
r
androstan-17beta-ol-3-one + NADH
-
Rattus norvegicus androstan-3alpha,17beta-diol + NAD+
-
?
androstan-3,17-dione + NADH
-
Rattus norvegicus androstan-3alpha-ol-17-one + NAD+
-
?
etiocholane-3,17-dione + NADH
-
Rattus norvegicus etiocholan-3alpha-ol-17-one + NAD+
-
?
pregnane-11beta,17alpha,21-triol-3,20-dione + NADH
-
Rattus norvegicus pregnane-3alpha,11beta,17alpha,21-tetrol-20-one + NAD+
-
?

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Rattus norvegicus
NADH
-
Rattus norvegicus
NADP+
-
Rattus norvegicus
NADPH
-
Rattus norvegicus