Inhibitors | Comment | Organism | Structure |
---|---|---|---|
21-hydroxypregn-4-ene-3,20-dione | - |
Mesocricetus auratus | |
5beta-Pregnan-3beta-ol-20-one | - |
Mesocricetus auratus | |
dexamethasone | - |
Mesocricetus auratus | |
Dienstrol | - |
Mesocricetus auratus | |
Hexestrol | - |
Mesocricetus auratus | |
Medroxyprogesterone acetate | - |
Mesocricetus auratus | |
p-chloromercuribenzenesulfonate | - |
Mesocricetus auratus | |
p-chloromercuribenzoate | - |
Mesocricetus auratus | |
stilboestrol | - |
Mesocricetus auratus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
NADP+ | - |
Mesocricetus auratus | |
0.005 | - |
NADPH | - |
Mesocricetus auratus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Mesocricetus auratus | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
gel filtration | Mesocricetus auratus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mesocricetus auratus | - |
golden hamster, adult, male | - |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, Q-Sepharose, Red A, HA-Ultrogel, Sephadex G-100, copurification with NAD+ dependent enzyme | Mesocricetus auratus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Mesocricetus auratus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.71 | - |
NADP+ dependent | Mesocricetus auratus |
4.2 | - |
NAD+ dependent | Mesocricetus auratus |
6.2 | - |
testis | Mesocricetus auratus |
9.8 | - |
kidney | Mesocricetus auratus |
26.8 | 375 | liver | Mesocricetus auratus |
Storage Stability | Organism |
---|---|
4°C, 10 mM-Tris/HCl, pH 8.0, 5 mM 2-mercaptoethanol, 0.5 mM EDTA, 20% glycerol | Mesocricetus auratus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3alpha-hydroxy-5alpha-androstan-17-one + NADP+ | - |
Mesocricetus auratus | 5alpha-androstan-3,17-dione + NADPH | - |
r | |
5alpha-androstan-17beta-ol-3-one + NADPH + H+ | - |
Mesocricetus auratus | 5alpha-androstan-3alpha,17beta-diol + NADP+ | - |
? | |
5alpha-androstan-3alpha-ol-17-one + NADH + H+ | - |
Mesocricetus auratus | 5beta-androstan-3alpha,17beta-diol + NAD+ | - |
? | |
5alpha-androstane-3alpha,17beta-diol + NADP+ | - |
Mesocricetus auratus | 5alpha-androstane-17beta-ol-3-one + NADPH | - |
r | |
5alpha-pregnan-3alpha,21-diol-20-one + NADP+ | - |
Mesocricetus auratus | 5alpha-pregnan-21-ol-3,20-dione + NADPH | - |
r | |
5beta-androstan-3alpha-ol-17-one + NADP+ | - |
Mesocricetus auratus | 5beta-androstan-3,17-dione + NADPH | - |
? | |
5beta-androstane-3alpha,17beta-diol + NADP+ | - |
Mesocricetus auratus | 5beta-androstane-17beta-ol-3-one + NADPH | - |
r | |
5beta-dihydrocortisone + NADPH | - |
Mesocricetus auratus | 3alpha,17alpha,21-trihydroxy-5beta-pregnan-11,20-dione + NADP+ | - |
? | |
5beta-pregnan-3alpha-ol-20-one + NADP+ | - |
Mesocricetus auratus | 5beta-pregnane-3,20-dione + NADPH | - |
r | |
benzenedihydrodiol + NAD(P)+ | - |
Mesocricetus auratus | ? | - |
? | |
glycochenodeoxycholic acid + NADP+ | - |
Mesocricetus auratus | 7alpha-hydroxy-3-oxo-5beta-cholanoyl glycine + NADPH | - |
? | |
glycolithocholic acid + NADP+ | - |
Mesocricetus auratus | 3-oxo-5beta-cholanoyl glycine + NADPH | - |
? | |
lithocholic acid + NADP+ | - |
Mesocricetus auratus | dehydrolithocholic acid + NADPH | - |
r |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 35000 SDS-PAGE | Mesocricetus auratus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Mesocricetus auratus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
50% inactivation after 17 min | Mesocricetus auratus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
glycochenodeoxycholic acid | - |
Mesocricetus auratus | |
0.217 | - |
lithocholic acid | - |
Mesocricetus auratus | |
0.233 | - |
glycolithocholic acid | - |
Mesocricetus auratus | |
0.233 | - |
5alpha-Pregnan-3alpha,21-diol-20-one | - |
Mesocricetus auratus | |
0.35 | - |
5alpha-androstan-17beta-ol-3-one | - |
Mesocricetus auratus | |
0.417 | - |
5beta-pregnan-3,20-dione | - |
Mesocricetus auratus | |
0.433 | - |
5beta-Dihydrocortisone | - |
Mesocricetus auratus | |
0.45 | - |
5beta-androstan-3,17-dione | - |
Mesocricetus auratus | |
0.567 | - |
5alpha-androstan-3,17-dione | - |
Mesocricetus auratus | |
0.633 | - |
5beta-Pregnan-3alpha,21-diol-20-one | - |
Mesocricetus auratus | |
0.733 | - |
Dehydrolithocholic acid | - |
Mesocricetus auratus | |
0.767 | - |
5beta-Pregnan-3alpha-ol-20-one | - |
Mesocricetus auratus | |
0.767 | - |
5alpha-pregnan-21-ol-3,20-one | - |
Mesocricetus auratus | |
1.2 | - |
5beta-Androstan-3alpha,17beta-diol | - |
Mesocricetus auratus | |
1.33 | - |
5beta-Androstan-3alpha-ol-17-one | - |
Mesocricetus auratus | |
1.33 | - |
5alpha-androstan-3alpha,17beta-diol | - |
Mesocricetus auratus | |
2.72 | - |
5alpha-Androstan-3alpha-ol-17-one | - |
Mesocricetus auratus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Mesocricetus auratus | |
NADPH | - |
Mesocricetus auratus |