Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.213 extracted from

  • Ohmura, M.; Hara, A.; Nakagawa, M.; Sawada, H.
    Demonstration of 3alpha(17beta)-hydroxysteroid dehydrogenase distinct from 3alpha-hydroxysteroid dehydrogenase in hamster liver (1990), Biochem. J., 266, 583-589.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
21-hydroxypregn-4-ene-3,20-dione
-
Mesocricetus auratus
5beta-Pregnan-3beta-ol-20-one
-
Mesocricetus auratus
dexamethasone
-
Mesocricetus auratus
Dienstrol
-
Mesocricetus auratus
Hexestrol
-
Mesocricetus auratus
Medroxyprogesterone acetate
-
Mesocricetus auratus
p-chloromercuribenzenesulfonate
-
Mesocricetus auratus
p-chloromercuribenzoate
-
Mesocricetus auratus
stilboestrol
-
Mesocricetus auratus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.003
-
NADP+
-
Mesocricetus auratus
0.005
-
NADPH
-
Mesocricetus auratus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Mesocricetus auratus 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
gel filtration Mesocricetus auratus

Organism

Organism UniProt Comment Textmining
Mesocricetus auratus
-
golden hamster, adult, male
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, Q-Sepharose, Red A, HA-Ultrogel, Sephadex G-100, copurification with NAD+ dependent enzyme Mesocricetus auratus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Mesocricetus auratus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.71
-
NADP+ dependent Mesocricetus auratus
4.2
-
NAD+ dependent Mesocricetus auratus
6.2
-
testis Mesocricetus auratus
9.8
-
kidney Mesocricetus auratus
26.8 375 liver Mesocricetus auratus

Storage Stability

Storage Stability Organism
4°C, 10 mM-Tris/HCl, pH 8.0, 5 mM 2-mercaptoethanol, 0.5 mM EDTA, 20% glycerol Mesocricetus auratus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3alpha-hydroxy-5alpha-androstan-17-one + NADP+
-
Mesocricetus auratus 5alpha-androstan-3,17-dione + NADPH
-
r
5alpha-androstan-17beta-ol-3-one + NADPH + H+
-
Mesocricetus auratus 5alpha-androstan-3alpha,17beta-diol + NADP+
-
?
5alpha-androstan-3alpha-ol-17-one + NADH + H+
-
Mesocricetus auratus 5beta-androstan-3alpha,17beta-diol + NAD+
-
?
5alpha-androstane-3alpha,17beta-diol + NADP+
-
Mesocricetus auratus 5alpha-androstane-17beta-ol-3-one + NADPH
-
r
5alpha-pregnan-3alpha,21-diol-20-one + NADP+
-
Mesocricetus auratus 5alpha-pregnan-21-ol-3,20-dione + NADPH
-
r
5beta-androstan-3alpha-ol-17-one + NADP+
-
Mesocricetus auratus 5beta-androstan-3,17-dione + NADPH
-
?
5beta-androstane-3alpha,17beta-diol + NADP+
-
Mesocricetus auratus 5beta-androstane-17beta-ol-3-one + NADPH
-
r
5beta-dihydrocortisone + NADPH
-
Mesocricetus auratus 3alpha,17alpha,21-trihydroxy-5beta-pregnan-11,20-dione + NADP+
-
?
5beta-pregnan-3alpha-ol-20-one + NADP+
-
Mesocricetus auratus 5beta-pregnane-3,20-dione + NADPH
-
r
benzenedihydrodiol + NAD(P)+
-
Mesocricetus auratus ?
-
?
glycochenodeoxycholic acid + NADP+
-
Mesocricetus auratus 7alpha-hydroxy-3-oxo-5beta-cholanoyl glycine + NADPH
-
?
glycolithocholic acid + NADP+
-
Mesocricetus auratus 3-oxo-5beta-cholanoyl glycine + NADPH
-
?
lithocholic acid + NADP+
-
Mesocricetus auratus dehydrolithocholic acid + NADPH
-
r

Subunits

Subunits Comment Organism
monomer 1 * 35000 SDS-PAGE Mesocricetus auratus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Mesocricetus auratus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
50% inactivation after 17 min Mesocricetus auratus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.2
-
glycochenodeoxycholic acid
-
Mesocricetus auratus
0.217
-
lithocholic acid
-
Mesocricetus auratus
0.233
-
glycolithocholic acid
-
Mesocricetus auratus
0.233
-
5alpha-Pregnan-3alpha,21-diol-20-one
-
Mesocricetus auratus
0.35
-
5alpha-androstan-17beta-ol-3-one
-
Mesocricetus auratus
0.417
-
5beta-pregnan-3,20-dione
-
Mesocricetus auratus
0.433
-
5beta-Dihydrocortisone
-
Mesocricetus auratus
0.45
-
5beta-androstan-3,17-dione
-
Mesocricetus auratus
0.567
-
5alpha-androstan-3,17-dione
-
Mesocricetus auratus
0.633
-
5beta-Pregnan-3alpha,21-diol-20-one
-
Mesocricetus auratus
0.733
-
Dehydrolithocholic acid
-
Mesocricetus auratus
0.767
-
5beta-Pregnan-3alpha-ol-20-one
-
Mesocricetus auratus
0.767
-
5alpha-pregnan-21-ol-3,20-one
-
Mesocricetus auratus
1.2
-
5beta-Androstan-3alpha,17beta-diol
-
Mesocricetus auratus
1.33
-
5beta-Androstan-3alpha-ol-17-one
-
Mesocricetus auratus
1.33
-
5alpha-androstan-3alpha,17beta-diol
-
Mesocricetus auratus
2.72
-
5alpha-Androstan-3alpha-ol-17-one
-
Mesocricetus auratus

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Mesocricetus auratus
NADPH
-
Mesocricetus auratus