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Literature summary for 1.1.1.21 extracted from
- Characterization and purification of an aldose from the acidophilic and thermophilic red algae Galdiera sulphuraria (1997), Plant Physiol., 114, 231-236.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 9.6 | - |
D-fructose | - |
Galdieria sulphuraria |  |
| 22 | - |
L-sorbose | - |
Galdieria sulphuraria | |
| 24.8 | - |
D-tagatose | - |
Galdieria sulphuraria | |
| 24.9 | - |
D-xylose-5-phosphate | - |
Galdieria sulphuraria | |
| 27 | - |
D-xylose | - |
Galdieria sulphuraria | |
| 29.1 | - |
D-ribose-5-phosphate | - |
Galdieria sulphuraria | |
| 42.9 | - |
D-galactose | - |
Galdieria sulphuraria | |
| 47.3 | - |
D-glucose | - |
Galdieria sulphuraria | |
| 52.3 | - |
L-rhamnose | - |
Galdieria sulphuraria | |
| 53.4 | - |
D-mannose | - |
Galdieria sulphuraria | |
| 59.3 | - |
D-fucose | - |
Galdieria sulphuraria | |
| 80.7 | - |
L-fucose | - |
Galdieria sulphuraria | |
| 120.5 | - |
hexanal | - |
Galdieria sulphuraria | |
| 245 | - |
L-ribose | - |
Galdieria sulphuraria | |
| 312.3 | - |
L-Lyxose | - |
Galdieria sulphuraria | |
| 370.9 | - |
D-ribose | - |
Galdieria sulphuraria | |
| 416.8 | - |
L-arabinose | - |
Galdieria sulphuraria | |
| 446.7 | - |
D-arabinose | - |
Galdieria sulphuraria | |
| 459.2 | - |
L-Xylose | - |
Galdieria sulphuraria | |
| 479.2 | - |
D-Lyxose | - |
Galdieria sulphuraria | |
| 730.2 | - |
DL-glyceraldehyde | - |
Galdieria sulphuraria | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
2 * 40000, SDS-PAGE | Galdieria sulphuraria |
| 80000 | - |
gel filtration | Galdieria sulphuraria |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pentose + NADPH + H+ | Galdieria sulphuraria | with hexoses and sugar phosphates as substrate the activity is 5-10% of that with pentoses | pentiol + NADP+ | - |
r | |
| pentose + NADPH + H+ | Galdieria sulphuraria | D-isoforms of pentoses show less than 10% activity compared to the L-isoforms | pentiol + NADP+ | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Galdieria sulphuraria | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Galdieria sulphuraria |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 17.1 | - |
purified enzyme | Galdieria sulphuraria |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-arabinose + NADPH | - |
Galdieria sulphuraria | arabitol + NADP+ | - |
r | |
| D-fructose + NAD(P)H + H+ | - |
Galdieria sulphuraria | D-arabino-hexitol + NAD(P)+ | - |
r | |
| D-fucose + NADPH | - |
Galdieria sulphuraria | fucitol + NADP+ | - |
r | |
| D-galactose + NAD(P)H | - |
Galdieria sulphuraria | dulcitol + NAD(P)+ | - |
r | |
| D-glucose + NAD(P)H | - |
Galdieria sulphuraria | sorbitol + NAD(P)+ | - |
r | |
| D-lyxose + NADPH | - |
Galdieria sulphuraria | arabitol + NADP+ | - |
r | |
| D-mannose + NAD(P)H | - |
Galdieria sulphuraria | mannitol + NAD(P)+ | - |
r | |
| D-ribose + NAD(P)H | - |
Galdieria sulphuraria | ribitol + NAD(P)+ | - |
r | |
| D-ribose + NADPH | - |
Galdieria sulphuraria | adonitol + NADP+ | - |
r | |
| D-ribose 5-phosphate + NADPH | - |
Galdieria sulphuraria | D-ribitol 5-phosphate + NADP+ | - |
r | |
| D-tagatose + NADPH | - |
Galdieria sulphuraria | ? | - |
r | |
| D-xylose + NAD(P)H | - |
Galdieria sulphuraria | xylitol + NAD(P)+ | - |
r | |
| D-xylose 5-phosphate + NADPH | - |
Galdieria sulphuraria | D-xylitol 5-phosphate + NADP+ | - |
r | |
| DL-glyceraldehyde + NAD(P)H | - |
Galdieria sulphuraria | glycerol + NAD(P)+ | - |
r | |
| hexanal + NADPH + H+ | - |
Galdieria sulphuraria | hexanol + NADP+ | - |
r | |
| L-arabinose + NAD(P)H | - |
Galdieria sulphuraria | L-arabitol + NAD(P)+ | - |
r | |
| L-arabinose + NADPH | - |
Galdieria sulphuraria | arabitol + NADP+ | - |
r | |
| L-fucose + NADPH | - |
Galdieria sulphuraria | fucitol + NADP+ | - |
r | |
| L-lyxose + NADPH | - |
Galdieria sulphuraria | arabitol + NADP+ | - |
r | |
| L-rhamnose + NADPH | - |
Galdieria sulphuraria | L-rhamnitol + NADP+ | - |
r | |
| L-ribose + NADPH | - |
Galdieria sulphuraria | adonitol + NADP+ | - |
r | |
| L-sorbose + NADPH + H+ | - |
Galdieria sulphuraria | L-sorbitol + NADP+ | - |
r | |
| L-xylose + NAD(P)H | - |
Galdieria sulphuraria | L-xylitol + NAD(P)+ | - |
r | |
| pentose + NADPH | with hexoses and sugar phosphates as substrate the activity was only 5-10% of that with pentoses | Galdieria sulphuraria | pentitol + NADP+ | - |
r | |
| pentose + NADPH + H+ | with hexoses and sugar phosphates as substrate the activity is 5-10% of that with pentoses | Galdieria sulphuraria | pentiol + NADP+ | - |
r | |
| pentose + NADPH + H+ | D-isoforms of pentoses show less than 10% activity compared to the L-isoforms | Galdieria sulphuraria | pentiol + NADP+ | - |
r | |
| xylitol + NAD(P)+ | - |
Galdieria sulphuraria | D-xylose + NAD(P)H + H+ | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 40000, SDS-PAGE | Galdieria sulphuraria |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
- |
Galdieria sulphuraria |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.8 | 7.6 | 59% activity at both threshold values | Galdieria sulphuraria |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | NADP(H) dependent, no activity with NAD(H) in either directions | Galdieria sulphuraria | |
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Release 2023.1 (January 2023)
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