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Literature summary for 1.1.1.209 extracted from

  • El-Kabbani, O.; Ishikura, S.; Wagner, A.; Schulze-Briese, C.; Hara, A.
    Crystallization and preliminary X-ray diffraction analysis of mouse 3(17)alpha-hydroxysteroid dehydrogenase (2005), Acta crystallogr. Sect. F, 61, 688-690.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression of isozyme AKR1C21 in Escherichia coli strain JM109 Mus musculus

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant isozyme AKR1C21, hanging drop vapour diffusion method, 16 mg/ml enzyme in 10 mM Tris-HCl, pH 7.5, and 2 mM 2-mercaptoethanol, mixed with an equal volume of 0.003 ml of reservoir solution containing 0.1 M HEPES, pH 7.5, 10% PEG 6000, 5% 2-methyl-2,4-pentanediol, equilibration against 1 ml reservoir solution, 22°C, 1 week, X-ray diffraction structure determination and analysis at 1.8 A resolution, molecular replacement Mus musculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39000
-
x * 39000, recombinant isozyme AKR1C21, SDS-PAGE Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus
-
isozyme AKR1C21
-

Purification (Commentary)

Purification (Comment) Organism
recombinant isozyme AKR1C21 from Escherichia coli strain JM109 to homogeneity Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
Mus musculus
-

Subunits

Subunits Comment Organism
? x * 39000, recombinant isozyme AKR1C21, SDS-PAGE Mus musculus

Synonyms

Synonyms Comment Organism
3(17)alpha-hydroxysteroid dehydrogenase
-
Mus musculus
AKR1C21
-
Mus musculus