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Literature summary for 1.1.1.208 extracted from

  • Akhtar, M.; Qamar, N.; Yadav, P.; Kulkarni, P.; Kumar, A.; Shasany, A.
    Comparative glandular trichome transcriptome-based gene characterization reveals reasons for differential (-)-menthol biosynthesis in Mentha species (2017), Physiol. Plant., 160, 128-141 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Mentha x piperita
expression in Escherichia coli Mentha arvensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0115
-
(-)-menthone pH 7, 30°C Mentha x piperita
0.024
-
(-)-menthone pH 7, 30°C Mentha arvensis
0.0467
-
(-)-menthone pH 7, 30°C Mentha arvensis
0.452
-
(-)-menthone pH 7, 30°C Mentha x piperita

Organism

Organism UniProt Comment Textmining
Mentha arvensis A0A1S5R240 isoform MD3
-
Mentha arvensis A0A1S5R244 isoform MD1
-
Mentha x piperita A0A1S5R238 isoform MD2
-
Mentha x piperita A0A1S5R263 isoform MD1
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(-)-menthone + NADPH + H+
-
Mentha x piperita (+)-neomenthol + NADP+
-
r
(-)-menthone + NADPH + H+
-
Mentha arvensis (+)-neomenthol + NADP+
-
r

Subunits

Subunits Comment Organism
? x * 16920, calculated from sequence Mentha arvensis
? x * 31095, calculated from sequence Mentha x piperita
? x * 34375, calculated from sequence Mentha arvensis
? x * 34590, calculated from sequence Mentha x piperita

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
46.8
-
(-)-menthone pH 7, 30°C Mentha arvensis
88.5
-
(-)-menthone pH 7, 30°C Mentha arvensis
89.6
-
(-)-menthone pH 7, 30°C Mentha x piperita
2588
-
(-)-menthone pH 7, 30°C Mentha x piperita

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1000
-
(-)-menthone pH 7, 30°C Mentha arvensis
3690
-
(-)-menthone pH 7, 30°C Mentha arvensis
5720
-
(-)-menthone pH 7, 30°C Mentha x piperita
7660
-
(-)-menthone pH 7, 30°C Mentha x piperita