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Literature summary for 1.1.1.208 extracted from

  • Choi, H.W.; Lee, B.G.; Kim, N.H.; Park, Y.; Lim, C.W.; Song, H.K.; Hwang, B.K.
    A role for a menthone reductase in resistance against microbial pathogens in plants (2008), Plant Physiol., 148, 383-401.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
additional information CaMNR1 is induced in leaves by bacterial infection and abiotic elicitor treatments. Under drought conditions, the CaMNR1 transcript is rapidly induced within 1 h and thereafter gradually increased to 25 h, while H2O2, cold and mechanical wounding treatment do not induce CaMNR1 expression in pepper leaves Capsicum annuum

Cloned(Commentary)

Cloned (Comment) Organism
gene AtSDR1, overexpression in Escherichia coli Arabidopsis thaliana
gene CaMNR1, DNA and amino acid sequence determination and analysis, phylogenetic tree, overexpression in Escherichia coli and in transgenic Arabidopsis thaliana plants Capsicum annuum

Protein Variants

Protein Variants Comment Organism
additional information AtSDR1 virus-induced gene silencing and ectopic overexpression in Arabidopsis thaliana plants leads to significantly more susceptible to Xanthomonas campestris pv. vesicatoria and Colletotrichum coccodes infection, and lower expression levels of salicylic acidresponsive CaBPR1 and CaPR10 and jasmonic acid-responsive CaDEF1 compared to wild-type plants, phenotype, overview Arabidopsis thaliana
additional information CaMNR1 tobacco rattle virus-mediated virus-induced gene silencing and ectopic overexpression in pepper and Arabidopsis thaliana plants, transgenic CaMNR1-silenced pepper plants are significantly more susceptible to Xanthomonas campestris pv. vesicatoria and Colletotrichum coccodes infection, and express lower levels of salicylic acidresponsive CaBPR1 and CaPR10 and jasmonic acid-responsive CaDEF1 compared to wild-type plants, while CaMNR1-overexpression transgenic Arabidopsis thaliana plants exhibit enhanced resistance to the hemi-biotrophic pathogen Pseudomonas syringae pv. tomato DC3000 and the biotrophic pathogen Hyaloperonospora parasitica isolate Noco2, accompanied by induction of AtPR1 and AtPDF1.2, phenotypes, overview Capsicum annuum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
gel filtration Capsicum annuum
34700
-
1 * 34700, about, sequence calculation Capsicum annuum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(-)-menthone + NADPH + H+ Arabidopsis thaliana
-
(+)-neomenthol + NADP+
-
r
(-)-menthone + NADPH + H+ Capsicum annuum in vitro antimicrobial activities of menthone, neomenthol and menthol, overview (3S)-neomenthol + NADP+
-
r
additional information Capsicum annuum CaMNR1 gene plays a role in regulating the resistance of pepper plants at the mRNA and protein levels, the menthone reductase gene CaMNR1 positively regulates plant defenses against a broad spectrum of pathogens, overview ?
-
?
additional information Arabidopsis thaliana the menthone reductase gene CaMNR1 positively regulates plant defenses against a broad spectrum of pathogens, overview ?
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9M2E2 pathogen responsive gene AtSDR1
-
Capsicum annuum B2X050 pathogen responsive gene CaMNR1
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli to homogeneity Capsicum annuum
recombinant enzyme from Escherichia coli to homogeneity Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
flower
-
Capsicum annuum
-
fruit in red, not in green, fruits Capsicum annuum
-
leaf the enzyme is induced in pepper leaves during compatible and incompatible interactions with Xcv, and by bacterial infection and abiotic elicitor treatments Capsicum annuum
-
additional information CaMNR1 is expressed in an organ-specific manner, no activity in uninduced leaves, stems, roots, or green fruits Capsicum annuum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(-)-menthone + NADPH + H+
-
Arabidopsis thaliana (+)-neomenthol + NADP+
-
r
(-)-menthone + NADPH + H+ in vitro antimicrobial activities of menthone, neomenthol and menthol, overview Capsicum annuum (3S)-neomenthol + NADP+
-
r
(-)-menthone + NADPH + H+ CaMNR1 converts (-)-menthone to 93% (3S)-neomenthol and 7% (3R)-menthol at pH 7.5, and to 72% (3S)-neomenthol and 28% (3R)-menthol at pH 9.0 with NADPH as a cofactor Capsicum annuum (3S)-neomenthol + NADP+
-
r
additional information CaMNR1 gene plays a role in regulating the resistance of pepper plants at the mRNA and protein levels, the menthone reductase gene CaMNR1 positively regulates plant defenses against a broad spectrum of pathogens, overview Capsicum annuum ?
-
?
additional information the menthone reductase gene CaMNR1 positively regulates plant defenses against a broad spectrum of pathogens, overview Arabidopsis thaliana ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 34700, about, sequence calculation Capsicum annuum

Synonyms

Synonyms Comment Organism
menthone:(+)-(3S)-neomenthol reductase
-
Capsicum annuum
menthone:(+)-(3S)-neomenthol reductase
-
Arabidopsis thaliana
More the enzyme is a member of the short chain dehydrogenase/reductase, SDR, superfamily Arabidopsis thaliana
More the enzyme is a member of the short chain dehydrogenase/reductase, SDR, superfamily, which adopts a unique alpha/beta structure known as the Rossmann fold Capsicum annuum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
the pH alters the stereospecificity of the enzyme Capsicum annuum
7.5
-
reduction reaction Capsicum annuum
9
-
oxidation reaction Capsicum annuum

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Capsicum annuum
NADPH the enzyme contains the coenzyme binding domain motif I: GxxxGxG Capsicum annuum

pI Value

Organism Comment pI Value Maximum pI Value
Capsicum annuum sequence calculation
-
5.39