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Literature summary for 1.1.1.206 extracted from

  • Bartholomew, B.A.; Smith, M.J.; Long, M.T.; Darcy, P.J.; Trudgill P.W.; Hopper, K.J.
    Tropine dehydrogenase: purification, some properties and an evaluation of its role in the bacterial metabolism of tropine (1995), Biochem. J., 307, 603-608.
No PubMed abstract available

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.006
-
Tropine
-
Pseudomonas sp.
0.048
-
NADP+
-
Pseudomonas sp.
0.073
-
nortropine
-
Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
SDS-PAGE Pseudomonas sp.
58000
-
gel filtration Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
tropine + NADP+ Pseudomonas sp. role in the pathway for tropine catabolism tropinone + NADPH
-
r
tropine + NADP+ Pseudomonas sp. AT3 role in the pathway for tropine catabolism tropinone + NADPH
-
r

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
AT3
-
Pseudomonas sp. AT3
-
AT3
-

Purification (Commentary)

Purification (Comment) Organism
-
Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.316
-
crude extract Pseudomonas sp.
63.82
-
purified enzyme Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nortropine + NADP+
-
Pseudomonas sp. nortropinone + NADPH
-
?
nortropine + NADP+
-
Pseudomonas sp. AT3 nortropinone + NADPH
-
?
tropine + NADP+
-
Pseudomonas sp. tropinone + NADPH
-
r
tropine + NADP+ role in the pathway for tropine catabolism Pseudomonas sp. tropinone + NADPH
-
r
tropine + NADP+
-
Pseudomonas sp. AT3 tropinone + NADPH
-
r
tropine + NADP+ role in the pathway for tropine catabolism Pseudomonas sp. AT3 tropinone + NADPH
-
r

Subunits

Subunits Comment Organism
dimer 2 identical subunits, gel filtration Pseudomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4
-
loss of 65% activity after 60 hours in crude extracts Pseudomonas sp.
4
-
loss of 90% activity of the purified enzyme after 10 hours, presence of 10% ethanol during the purification procedure Pseudomonas sp.

Cofactor

Cofactor Comment Organism Structure
additional information no activity with NAD+ Pseudomonas sp.
NADP+
-
Pseudomonas sp.