Cloned (Comment) | Organism |
---|---|
gene guaB, recombinant overexpression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Bacillus amyloliquefaciens |
Protein Variants | Comment | Organism |
---|---|---|
K481E | site-directed mutagenesis, site 481 is a key mutation site to affect the NAD+ affinity, which accounts for the higher catalytic efficiency of the IMPDH mutant compared to the wild-type enzyme | Bacillus amyloliquefaciens |
additional information | construction of a deletion mutant IMPDHDELTACBS lacking the CBS domains | Bacillus amyloliquefaciens |
P166S | site-directed mutagenesis, the mutant shows unaltered catalytic activity compared to the wild-type enzyme | Bacillus amyloliquefaciens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
IMP | pH 8.0, 40°C, recombinant wild-type enzyme and mutant P166S and K481E | Bacillus amyloliquefaciens | |
0.013 | - |
IMP | pH 8.0, 40°C, recombinant mutant enzyme IMPDHDELTACBS | Bacillus amyloliquefaciens | |
2.7 | - |
NAD+ | pH 8.0, 40°C, recombinant mutant K481E | Bacillus amyloliquefaciens | |
3.1 | - |
NAD+ | pH 8.0, 40°C, recombinant wild-type enzyme and mutant IMPDHDELTACBS | Bacillus amyloliquefaciens | |
3.2 | - |
NAD+ | pH 8.0, 40°C, recombinant mutant P166S | Bacillus amyloliquefaciens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
IMP + NAD+ + H2O | Bacillus amyloliquefaciens | - |
XMP + NADH + H+ | - |
? | |
IMP + NAD+ + H2O | Bacillus amyloliquefaciens TA208 | - |
XMP + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus amyloliquefaciens | D1MF29 | a guanosine overproducing strain | - |
Bacillus amyloliquefaciens TA208 | D1MF29 | a guanosine overproducing strain | - |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant enzymes from Escherichia coli strain Bl21(DE3) | Bacillus amyloliquefaciens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
IMP + NAD+ + H2O | - |
Bacillus amyloliquefaciens | XMP + NADH + H+ | - |
? | |
IMP + NAD+ + H2O | - |
Bacillus amyloliquefaciens TA208 | XMP + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
IMPDH | - |
Bacillus amyloliquefaciens |
inosine monophosphate dehydrogenase | - |
Bacillus amyloliquefaciens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Bacillus amyloliquefaciens |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | 55 | activity range, profile overview | Bacillus amyloliquefaciens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4 | - |
NAD+ | pH 8.0, 40°C, recombinant wild-type enzyme and mutant K481E | Bacillus amyloliquefaciens | |
5 | - |
IMP | pH 8.0, 40°C, recombinant mutant enzymes, P166S, K481E, and IMPDHDELTACBS | Bacillus amyloliquefaciens | |
5.1 | - |
IMP | pH 8.0, 40°C, recombinant wild-type enzyme | Bacillus amyloliquefaciens | |
15 | - |
NAD+ | pH 8.0, 40°C, recombinant wild-type enzyme and mutant P166S | Bacillus amyloliquefaciens | |
16 | - |
NAD+ | pH 8.0, 40°C, recombinant mutant enzyme IMPDHDELTACBS | Bacillus amyloliquefaciens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Bacillus amyloliquefaciens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | activity range, profile overview | Bacillus amyloliquefaciens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Bacillus amyloliquefaciens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.6 | - |
NAD+ | pH 8.0, 40°C, recombinant mutant K481E | Bacillus amyloliquefaciens | |
4.6 | - |
NAD+ | pH 8.0, 40°C, recombinant mutant P166S | Bacillus amyloliquefaciens | |
4.9 | - |
NAD+ | pH 8.0, 40°C, recombinant wild-type enzyme | Bacillus amyloliquefaciens | |
5.2 | - |
NAD+ | pH 8.0, 40°C, recombinant mutant IMPDHDELTACBS | Bacillus amyloliquefaciens | |
40 | 43 | IMP | pH 8.0, 40°C, recombinant wild-type enzyme, and recombinant mutant enzymes, P166S, K481E, and IMPDHDELTACBS | Bacillus amyloliquefaciens |