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Literature summary for 1.1.1.205 extracted from

  • Wang, J.; He, K.; Xu, Q.; Chen, N.
    Mutagenetic study of a novel inosine monophosphate dehydrogenase from Bacillus amyloliquefaciens and its possible application in guanosine production (2014), Biotechnol. Biotechnol. Equip., 28, 102-106.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene guaB, recombinant overexpression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Bacillus amyloliquefaciens

Protein Variants

Protein Variants Comment Organism
K481E site-directed mutagenesis, site 481 is a key mutation site to affect the NAD+ affinity, which accounts for the higher catalytic efficiency of the IMPDH mutant compared to the wild-type enzyme Bacillus amyloliquefaciens
additional information construction of a deletion mutant IMPDHDELTACBS lacking the CBS domains Bacillus amyloliquefaciens
P166S site-directed mutagenesis, the mutant shows unaltered catalytic activity compared to the wild-type enzyme Bacillus amyloliquefaciens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.012
-
IMP pH 8.0, 40°C, recombinant wild-type enzyme and mutant P166S and K481E Bacillus amyloliquefaciens
0.013
-
IMP pH 8.0, 40°C, recombinant mutant enzyme IMPDHDELTACBS Bacillus amyloliquefaciens
2.7
-
NAD+ pH 8.0, 40°C, recombinant mutant K481E Bacillus amyloliquefaciens
3.1
-
NAD+ pH 8.0, 40°C, recombinant wild-type enzyme and mutant IMPDHDELTACBS Bacillus amyloliquefaciens
3.2
-
NAD+ pH 8.0, 40°C, recombinant mutant P166S Bacillus amyloliquefaciens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
IMP + NAD+ + H2O Bacillus amyloliquefaciens
-
XMP + NADH + H+
-
?
IMP + NAD+ + H2O Bacillus amyloliquefaciens TA208
-
XMP + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Bacillus amyloliquefaciens D1MF29 a guanosine overproducing strain
-
Bacillus amyloliquefaciens TA208 D1MF29 a guanosine overproducing strain
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and mutant enzymes from Escherichia coli strain Bl21(DE3) Bacillus amyloliquefaciens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
IMP + NAD+ + H2O
-
Bacillus amyloliquefaciens XMP + NADH + H+
-
?
IMP + NAD+ + H2O
-
Bacillus amyloliquefaciens TA208 XMP + NADH + H+
-
?

Synonyms

Synonyms Comment Organism
IMPDH
-
Bacillus amyloliquefaciens
inosine monophosphate dehydrogenase
-
Bacillus amyloliquefaciens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Bacillus amyloliquefaciens

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
15 55 activity range, profile overview Bacillus amyloliquefaciens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4
-
NAD+ pH 8.0, 40°C, recombinant wild-type enzyme and mutant K481E Bacillus amyloliquefaciens
5
-
IMP pH 8.0, 40°C, recombinant mutant enzymes, P166S, K481E, and IMPDHDELTACBS Bacillus amyloliquefaciens
5.1
-
IMP pH 8.0, 40°C, recombinant wild-type enzyme Bacillus amyloliquefaciens
15
-
NAD+ pH 8.0, 40°C, recombinant wild-type enzyme and mutant P166S Bacillus amyloliquefaciens
16
-
NAD+ pH 8.0, 40°C, recombinant mutant enzyme IMPDHDELTACBS Bacillus amyloliquefaciens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Bacillus amyloliquefaciens

pH Range

pH Minimum pH Maximum Comment Organism
6 9 activity range, profile overview Bacillus amyloliquefaciens

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Bacillus amyloliquefaciens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.6
-
NAD+ pH 8.0, 40°C, recombinant mutant K481E Bacillus amyloliquefaciens
4.6
-
NAD+ pH 8.0, 40°C, recombinant mutant P166S Bacillus amyloliquefaciens
4.9
-
NAD+ pH 8.0, 40°C, recombinant wild-type enzyme Bacillus amyloliquefaciens
5.2
-
NAD+ pH 8.0, 40°C, recombinant mutant IMPDHDELTACBS Bacillus amyloliquefaciens
40 43 IMP pH 8.0, 40°C, recombinant wild-type enzyme, and recombinant mutant enzymes, P166S, K481E, and IMPDHDELTACBS Bacillus amyloliquefaciens