Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Trypanosoma brucei |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
mizoribine 5'-monophosphate | - |
Trypanosoma brucei | |
Mycophenolic acid | - |
Trypanosoma brucei | |
NAD+ | substrate inhibition | Trypanosoma brucei | |
ribavirin 5'-monophosphate | - |
Trypanosoma brucei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
Inosine 5'-diphosphate | pH 8.0, 25°C | Trypanosoma brucei | |
1.3 | - |
NAD+ | pH 8.0, 25°C | Trypanosoma brucei |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57000 | - |
7 * 57000, SDS-PAGE | Trypanosoma brucei |
389000 | - |
sedimentation velocity analysis | Trypanosoma brucei |
420000 | - |
gel fitlration | Trypanosoma brucei |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
inosine 5'-diphosphate + NAD+ + H2O | Trypanosoma brucei | - |
xanthosine 5'-diphosphate + NADH + H+ | xanthosine 5'-diphosphate is the only product | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma brucei | Q387Q3 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
inosine 5'-diphosphate + NAD+ + H2O | - |
Trypanosoma brucei | xanthosine 5'-diphosphate + NADH + H+ | xanthosine 5'-diphosphate is the only product | ? |
Subunits | Comment | Organism |
---|---|---|
heptamer | 7 * 57000, SDS-PAGE | Trypanosoma brucei |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.28 | - |
Inosine 5'-diphosphate | pH 8.0, 25°C | Trypanosoma brucei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Trypanosoma brucei |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Trypanosoma brucei |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0032 | - |
ribavirin 5'-monophosphate | pH 8.0, 25°C | Trypanosoma brucei | |
0.0033 | - |
mizoribine 5'-monophosphate | pH 8.0, 25°C | Trypanosoma brucei | |
0.021 | - |
Mycophenolic acid | pH 8.0, 25°C | Trypanosoma brucei | |
3 | - |
NAD+ | pH 8.0, 25°C | Trypanosoma brucei |