Literature summary for 1.1.1.205 extracted from

Zhang, X.H.; He, K.W.; Duan, Z.T.; Zhou, J.M.; Yu, Z.Y.; Ni, Y.X.; Lu, C.P.
Identification and characterization of inosine 5-monophosphate dehydrogenase in Streptococcus suis type 2 (2009), Microb. Pathog., 7, 267-273.

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Cloned(Commentary)

Cloned (Comment)	Organism
pET32 expression plasmid, from which the impdh gene is inducibly overexpressed in Escherichia coli BL21 (DE3) to produce IMPDH with a hexahistidyl N-terminus	Streptococcus suis

Protein Variants

Protein Variants	Comment	Organism
additional information	impdh-knockout mutant (DELTAIMPDH) is slower in growth and one pH unit higher than the parent after 6 h of culturing. DELTAIMPDH is negative for fermentation maltose, salicin, lactose, raffinose, mannose and glucose metabolism in contrast to the parent strain, but it can fermentate esculin, nitrate and glucosamine like the parent. The mutant is attenuated in mouse models of infection for 2.5times and not be capable of causing death in porcine models of infection in contrast with the parent	Streptococcus suis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
52200	-	(His)6 IMPDH protein, Western blot analysis	Streptococcus suis

Organism

Organism	UniProt	Comment	Textmining
Streptococcus suis	-	type 2 strain	-
Streptococcus suis 2	-	type 2 strain	-

Purification (Commentary)

Purification (Comment)	Organism
by Ni-NTA affinity chromatography	Streptococcus suis

Storage Stability

Storage Stability	Organism
-20°C	Streptococcus suis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
inosine 5'-phosphate + NAD+ + H2O	-	Streptococcus suis	xanthosine 5'-phosphate + NADH + H+	-	?
inosine 5'-phosphate + NAD+ + H2O	-	Streptococcus suis 2	xanthosine 5'-phosphate + NADH + H+	-	?

Synonyms

Synonyms	Comment	Organism
IMPDH	-	Streptococcus suis
inosine 5-monophosphate dehydrogenase	-	Streptococcus suis

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	Streptococcus suis

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Release 2023.1 (January 2023)