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Literature summary for 1.1.1.205 extracted from

  • Ji, Y.; Gu, J.; Makhov, A.M.; Griffith, J.D.; Mitchell, B.S.
    Regulation of the interaction of inosine monophosphate dehydrogenase with mycophenolic acid by GTP (2006), J. Biol. Chem., 281, 206-212.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
ATP allosteric activator of the enzyme Homo sapiens

Application

Application Comment Organism
medicine the enzyme is a major therapeutic target Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression of His-tagged type II IMPDH in Escherichia coli strain BL21(DE3) Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Mycophenolic acid uncompetitive inhibition, the interaction with the enzyme, inducing striking conformational changes and aggregation, is regulated by GTP, GTP reverses the enzyme aggregation by inhibitor mycophenolic acid, the inhibitor is part of an immunosuppressive drug Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens the enzyme is rate-limiting in the de novo synthesis of guanine nucleotides ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
type II IMPDH isozyme
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme from MCF7 cells partially by gel filtration, recombinant His-tagged type II IMPDH from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
CEM cell HGPRT-deficient acute lymphoblastic leukemia cell line Homo sapiens
-
K6H6/B5 cell HGPRT-deficient hybridoma cell line Homo sapiens
-
MCF7 cell breast cancer cell line Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme is rate-limiting in the de novo synthesis of guanine nucleotides Homo sapiens ?
-
?
additional information the enzyme interacts with GTP which leads to formation of linear arrays, GTP reverses the enzyme aggregation by inhibitor mycophenolic acid Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
IMPDH
-
Homo sapiens
inosine monophosphate dehydrogenase
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Homo sapiens