Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in enzyme-deficient Escherichia coli strain H712 | Tritrichomonas suis |
Protein Variants | Comment | Organism |
---|---|---|
R418A | site-directed mutagenesis, altered kinetics compared to the wild-type enzyme, chemical rescue of the mutant by guanidine, methylguanidine, ethylguanidine, acteamide, and aminoguanidine, not by hydroxyurea, overview | Tritrichomonas suis |
R418A/Y419F | site-directed mutagenesis, altered kinetics compared to the wild-type enzyme, no rescue of the mutant by aminoguanidine, overview | Tritrichomonas suis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics, wild-type enzyme and mutant enzymes | Tritrichomonas suis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | 100-800 mM K+ does not affect the R418A mutant activity | Tritrichomonas suis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
inosine 5'-phosphate + NAD+ + H2O | Tritrichomonas suis | - |
xanthosine 5'-phosphate + NADH | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Tritrichomonas suis | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
IMP + NAD+ + H2O = XMP + NADH + H+ | Arg418 is involved in the reaction mechanism as catalytic base | Tritrichomonas suis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
inosine 5'-phosphate + NAD+ + H2O | - |
Tritrichomonas suis | xanthosine 5'-phosphate + NADH | - |
r |
Synonyms | Comment | Organism |
---|---|---|
IMPDH | - |
Tritrichomonas suis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Tritrichomonas suis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Tritrichomonas suis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Tritrichomonas suis | |
NADH | - |
Tritrichomonas suis |