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Literature summary for 1.1.1.205 extracted from
- IMP dehydrogenase from the protozoan parasite Toxoplasma gondii (2005), Antimicrob. Agents Chemother., 49, 2172-2179.
Application
| Application | Comment | Organism |
|---|---|---|
| drug development | the enzyme is a target in parasite treatment and elimination | Toxoplasma gondii |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis, two splicing variants, a short and a large one, overview | Toxoplasma gondii |
| DNA and amino acid sequence determination and analysis, two splicing variants, a short and a large one, overview, expression of FLAG-tagged large splicing variant in the parasite with localization in the cytoplasm | Toxoplasma gondii |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CH2-SAD | i.e. 4-carboxamido-2-beta-D-ribofuranosylselenazolyl adenosine methylenediphosphonic acid, an adenine dinucleotide analogue nonhydrolyzable beta-methylene derivative | Toxoplasma gondii |  |
| CH2-TAD | i.e. 4-carboxamido-2-beta-D-ribofuranosylthiazolyl adenosine methylenediphosphonic acid, an adenine dinucleotide analogue nonhydrolyzable beta-methylene derivative | Toxoplasma gondii | |
| inosine 5'-phosphate | - |
Toxoplasma gondii | |
| additional information | no inhibition by tiazofurin and seleno-thiazofurin | Toxoplasma gondii | |
| Mycophenolic acid | uncompetitive to NAD+ | Toxoplasma gondii | |
| NAD+ | - |
Toxoplasma gondii | |
| SAD | i.e. selenazole-4-carboxamido adenine diphosphonic acid, an adenine dinucleotide analogue | Toxoplasma gondii | |
| TAD | i.e. thiazole-4-carboxamide adenine dinucleotide, an adenine dinucleotide analogue | Toxoplasma gondii | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.144 | - |
NAD+ | pH 8.0, 37°C, recombinant enzyme | Toxoplasma gondii | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| inosine 5'-phosphate + NAD+ + H2O | Toxoplasma gondii | - |
xanthosine 5'-phosphate + NADH | - |
? | |
| additional information | Toxoplasma gondii | the parasite damages fetuses in utero and threatens immunocompromized individuals | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Toxoplasma gondii | Q4VRV8 | large splicing variant IMPDH; strains RH and RHDELTATHX, opportunistic apicomplexan parasite, two splicing variants, a short and a large one | - |
| Toxoplasma gondii | Q4VRV8 | short splicing variant IMPDH-S; strains RH and RHDELTATHX, opportunistiuc apicomplexan parasite, two splicing variants, a short and a large one | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant FLAG-tagged large splicing variant by immunoaffinity | Toxoplasma gondii |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| tachyzoite | cultured in primary human foreskin fibroblasts | Toxoplasma gondii | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Toxoplasma gondii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| inosine 5'-phosphate + NAD+ + H2O | - |
Toxoplasma gondii | xanthosine 5'-phosphate + NADH | - |
? | |
| additional information | the parasite damages fetuses in utero and threatens immunocompromized individuals | Toxoplasma gondii | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| IMPDH | - |
Toxoplasma gondii |
| IMPDH-S | - |
Toxoplasma gondii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Toxoplasma gondii |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.98 | - |
NAD+ | pH 8.0, 37°C, recombinant enzyme | Toxoplasma gondii | |
| 0.98 | - |
inosine 5'-phosphate | pH 8.0, 37°C, recombinant enzyme | Toxoplasma gondii | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Toxoplasma gondii |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Toxoplasma gondii | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00003 | - |
Mycophenolic acid | pH 8.0, 37°C, recombinant enzyme | Toxoplasma gondii | |
| 0.00038 | - |
CH2-SAD | pH 8.0, 37°C, recombinant enzyme | Toxoplasma gondii | |
| 0.00098 | - |
CH2-TAD | pH 8.0, 37°C, recombinant enzyme | Toxoplasma gondii | |
| 0.0018 | - |
TAD | pH 8.0, 37°C, recombinant enzyme | Toxoplasma gondii | |
| 0.0018 | - |
SAD | pH 8.0, 37°C, recombinant enzyme | Toxoplasma gondii | |
| 4.5 | - |
inosine 5'-phosphate | pH 8.0, 37°C, recombinant enzyme | Toxoplasma gondii | |
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