Cloned (Comment) | Organism |
---|---|
- |
Streptococcus pyogenes |
Crystallization (Comment) | Organism |
---|---|
vapor diffusion method using hanging drops | Streptococcus pyogenes |
Protein Variants | Comment | Organism |
---|---|---|
E421Q | full enzymatic activity | Streptococcus pyogenes |
R406A | no enzymatic activity | Streptococcus pyogenes |
Y450A | 25% of enzymatic activity | Streptococcus pyogenes |
Y450D | no enzymatic activity | Streptococcus pyogenes |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
6-Chloropurine | irreversible inhibitor | Streptococcus pyogenes | |
mizobirine 5'-monophosphate | - |
Streptococcus pyogenes | |
Mycophenolic acid | - |
Streptococcus pyogenes | |
ribavirin 5'-monophosphate | - |
Streptococcus pyogenes |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.062 | - |
IMP | - |
Streptococcus pyogenes | |
1.18 | - |
NAD+ | - |
Streptococcus pyogenes |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | - |
Streptococcus pyogenes | |
additional information | influence of various metal ions on enzyme activity | Streptococcus pyogenes |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52330 | - |
MALDI-MS | Streptococcus pyogenes |
52700 | - |
SDS-PAGE | Streptococcus pyogenes |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus pyogenes | P0C0H6 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Streptococcus pyogenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
inosine 5'-phosphate + NAD+ + H2O | - |
Streptococcus pyogenes | xanthosine 5'-phosphate + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | MAD analysis | Streptococcus pyogenes |
tetramer | - |
Streptococcus pyogenes |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.4 | - |
IMP | - |
Streptococcus pyogenes |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
- |
Streptococcus pyogenes |