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Literature summary for 1.1.1.205 extracted from

  • Zhang, R.G.; Evans, G.; Rotella, F.J.; Westbrook, E.M.; Beno, D.; Huberman, E; Joachimiak, A.; Collart, F.R.
    Characteristics and crystal structure of bacterial inosine-5'-monophosphate dehydrogenase (1999), Biochemistry, 38, 4691-4700.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Streptococcus pyogenes

Crystallization (Commentary)

Crystallization (Comment) Organism
vapor diffusion method using hanging drops Streptococcus pyogenes

Protein Variants

Protein Variants Comment Organism
E421Q full enzymatic activity Streptococcus pyogenes
R406A no enzymatic activity Streptococcus pyogenes
Y450A 25% of enzymatic activity Streptococcus pyogenes
Y450D no enzymatic activity Streptococcus pyogenes

Inhibitors

Inhibitors Comment Organism Structure
6-Chloropurine irreversible inhibitor Streptococcus pyogenes
mizobirine 5'-monophosphate
-
Streptococcus pyogenes
Mycophenolic acid
-
Streptococcus pyogenes
ribavirin 5'-monophosphate
-
Streptococcus pyogenes

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.062
-
IMP
-
Streptococcus pyogenes
1.18
-
NAD+
-
Streptococcus pyogenes

Metals/Ions

Metals/Ions Comment Organism Structure
K+
-
Streptococcus pyogenes
additional information influence of various metal ions on enzyme activity Streptococcus pyogenes

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52330
-
MALDI-MS Streptococcus pyogenes
52700
-
SDS-PAGE Streptococcus pyogenes

Organism

Organism UniProt Comment Textmining
Streptococcus pyogenes P0C0H6
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Streptococcus pyogenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
inosine 5'-phosphate + NAD+ + H2O
-
Streptococcus pyogenes xanthosine 5'-phosphate + NADH + H+
-
?

Subunits

Subunits Comment Organism
? MAD analysis Streptococcus pyogenes
tetramer
-
Streptococcus pyogenes

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.4
-
IMP
-
Streptococcus pyogenes

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
-
Streptococcus pyogenes