Cloned (Comment) | Organism |
---|---|
udh gene from genomic DNA amplified and inserted into pET21b containing a six-His tag and expressed in Escherichia coli BL21(DE3) or inserted into vector pTrc99SE and transformed into Escherichia coli mutant strain MG1655 DELTAuxaC | Pseudomonas syringae pv. tomato |
udh gene from genomic DNA amplified and inserted into pET21b containing a six-His tag and expressed in Escherichia coli BL21(DE3) or inserted into vector pTrc99SE and transformed into Escherichia coli mutant strain MG1655 DELTAuxaC | Agrobacterium tumefaciens |
udh gene from genomic DNA amplified and inserted into pET21b containing a six-His tag and expressed in Escherichia coli BL21(DE3) or inserted into vector pTrc99SE and transformed into Escherichia coli mutant strain MG1655 DELTAuxaC | Pseudomonas putida KT2440 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
D-galacturonate | - |
Pseudomonas syringae pv. tomato | |
0.1 | - |
D-galacturonate | - |
Pseudomonas putida KT2440 | |
0.16 | - |
D-galacturonate | - |
Agrobacterium tumefaciens | |
0.17 | - |
NAD+ | - |
Pseudomonas syringae pv. tomato | |
0.18 | - |
NAD+ | - |
Agrobacterium tumefaciens | |
0.21 | - |
NAD+ | - |
Pseudomonas putida KT2440 | |
0.25 | - |
D-glucuronate | - |
Pseudomonas putida KT2440 | |
0.28 | - |
D-glucuronate | - |
Pseudomonas syringae pv. tomato | |
0.37 | - |
D-glucuronate | - |
Agrobacterium tumefaciens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
x * 30000, SDS-PAGE | Pseudomonas syringae pv. tomato |
30000 | - |
x * 30000, SDS-PAGE | Pseudomonas putida KT2440 |
32000 | - |
x * 32000, SDS-PAGE | Agrobacterium tumefaciens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Agrobacterium tumefaciens | Q7CRQ0 | - |
- |
Agrobacterium tumefaciens C58 / ATCC 33970 | Q7CRQ0 | - |
- |
Pseudomonas putida KT2440 | Q88NN6 | - |
- |
Pseudomonas syringae pv. tomato | Q888H1 | - |
- |
Pseudomonas syringae pv. tomato DC3000 | Q888H1 | - |
- |
Purification (Comment) | Organism |
---|---|
gel filtration | Pseudomonas syringae pv. tomato |
gel filtration | Agrobacterium tumefaciens |
gel filtration | Pseudomonas putida KT2440 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-galacturonate + NAD+ + H2O | - |
Pseudomonas syringae pv. tomato | D-galactarate + NADH + H+ | - |
? | |
D-galacturonate + NAD+ + H2O | - |
Agrobacterium tumefaciens | D-galactarate + NADH + H+ | - |
? | |
D-galacturonate + NAD+ + H2O | - |
Pseudomonas putida KT2440 | D-galactarate + NADH + H+ | - |
? | |
D-galacturonate + NAD+ + H2O | - |
Pseudomonas syringae pv. tomato DC3000 | D-galactarate + NADH + H+ | - |
? | |
D-galacturonate + NAD+ + H2O | - |
Agrobacterium tumefaciens C58 / ATCC 33970 | D-galactarate + NADH + H+ | - |
? | |
D-glucuronate + NAD+ + H2O | - |
Pseudomonas syringae pv. tomato | D-glucaric acid + NADH + H+ | - |
? | |
D-glucuronate + NAD+ + H2O | - |
Agrobacterium tumefaciens | D-glucaric acid + NADH + H+ | - |
? | |
D-glucuronate + NAD+ + H2O | - |
Pseudomonas putida KT2440 | D-glucaric acid + NADH + H+ | - |
? | |
D-glucuronate + NAD+ + H2O | - |
Pseudomonas syringae pv. tomato DC3000 | D-glucaric acid + NADH + H+ | - |
? | |
D-glucuronate + NAD+ + H2O | - |
Agrobacterium tumefaciens C58 / ATCC 33970 | D-glucaric acid + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 32000, SDS-PAGE | Agrobacterium tumefaciens |
? | x * 30000, SDS-PAGE | Pseudomonas syringae pv. tomato |
? | x * 30000, SDS-PAGE | Pseudomonas putida KT2440 |
Synonyms | Comment | Organism |
---|---|---|
Udh | - |
Pseudomonas syringae pv. tomato |
Udh | - |
Agrobacterium tumefaciens |
Udh | - |
Pseudomonas putida KT2440 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.24 | - |
D-galacturonate | - |
Pseudomonas syringae pv. tomato | |
0.3 | - |
D-galacturonate | - |
Pseudomonas putida KT2440 | |
0.55 | - |
D-glucuronate | - |
Pseudomonas putida KT2440 | |
0.74 | - |
D-glucuronate | - |
Pseudomonas syringae pv. tomato | |
0.92 | - |
D-galacturonate | - |
Agrobacterium tumefaciens | |
1.9 | - |
D-glucuronate | - |
Agrobacterium tumefaciens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Pseudomonas putida KT2440 |
8 | - |
- |
Pseudomonas syringae pv. tomato |
8 | - |
- |
Agrobacterium tumefaciens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Pseudomonas syringae pv. tomato | |
NAD+ | - |
Agrobacterium tumefaciens | |
NAD+ | - |
Pseudomonas putida KT2440 |