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Literature summary for 1.1.1.202 extracted from

  • Ma, Z.; Rao, Z.; Zhuge, B.; Fang, H.; Liao, X.; Zhuge, J.
    Construction of a Novel Expression System in Klebsiella pneumoniae and its Application for 1,3-Propanediol Production (2010), Appl. Biochem. Biotechnol., 162, 399-407.
    View publication on PubMed

Application

Application Comment Organism
synthesis constitutive overexpression of 1,3-PD oxidoreductase in Klebsiella pneumoniae leads to a nearly 3fold decrease in peak level of the intermediary metabolite 3-hydroxypropionaldehyde and an increase of 16.5% in yield of 1,3-propanediol with respect to the wild-type strain Klebsiella pneumoniae

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of gene dhaT encoding PDOR (from vector pETPkan-dhaT) and transformed into Klebsiella pneumoniae Klebsiella pneumoniae

Organism

Organism UniProt Comment Textmining
Klebsiella pneumoniae
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-hydroxypropanal + NADH + H+ level of PDOR activity of Klebsiella pneumoniae/pETPkan-dhaT (1.64 U/mg) shows an increase of 0.9fold in PDOR activity with respect to the wild-type Klebsiella pneumoniae (0.85 U/mg). The recombinant strain Klebsiella pneumoniae/pETPkan-dhaT improves propane-1,3-diol production by 16.5% with respect to the wild-type strain Klebsiella pneumoniae propane-1,3-diol + NAD+
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Synonyms

Synonyms Comment Organism
(NADH)-linked 1,3-PD oxidoreductase
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Klebsiella pneumoniae
PDOR
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Klebsiella pneumoniae