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Literature summary for 1.1.1.201 extracted from

  • Zheng, M.M.; Chen, K.C.; Wang, R.F.; Li, H.; Li, C.X.; Xu, J.H.
    Engineering 7beta-hydroxysteroid dehydrogenase for enhanced ursodeoxycholic acid production by multiobjective directed evolution (2017), J. Agric. Food Chem., 65, 1178-1185 .
    View publication on PubMed
Search for more literature for 1.1.1.201

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells [Ruminococcus] torques

Protein Variants

Protein Variants Comment Organism
G57S/I163V/V207M the mutant shows increased catalytic efficiency compared to the wild type enzyme [Ruminococcus] torques
I112V/N240D the mutant shows increased catalytic efficiency compared to the wild type enzyme [Ruminococcus] torques
I112V/T189I/N240D the mutant shows strongly decreased catalytic efficiency compared to the wild type enzyme [Ruminococcus] torques
I28V/V38A/I76V/V207M the mutant shows increased catalytic efficiency compared to the wild type enzyme [Ruminococcus] torques
K44E/T189I the mutant shows decreased catalytic efficiency compared to the wild type enzyme [Ruminococcus] torques
N240I the mutant shows increased catalytic efficiency compared to the wild type enzyme [Ruminococcus] torques
T189V the mutant shows increased catalytic efficiency compared to the wild type enzyme [Ruminococcus] torques
T189V/V207M the mutant shows increased catalytic efficiency compared to the wild type enzyme [Ruminococcus] torques
V207M the mutant shows increased catalytic efficiency compared to the wild type enzyme [Ruminococcus] torques

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0078
-
 NADPH wild type enzyme, at pH 8.0 and 30°C [Ruminococcus] torques
0.021
-
 7-oxo-lithocholic acid mutant enzyme I28V/V38A/I76V/V207M, at pH 8.0 and 30°C [Ruminococcus] torques
0.023
-
 7-oxo-lithocholic acid wild type enzyme, at pH 8.0 and 30°C [Ruminococcus] torques
0.023
-
 7-oxo-lithocholic acid mutant enzyme N240I, at pH 8.0 and 30°C [Ruminococcus] torques
0.028
-
 7-oxo-lithocholic acid mutant enzyme I112V/N240D, at pH 8.0 and 30°C [Ruminococcus] torques
0.031
-
 7-oxo-lithocholic acid mutant enzyme T189V, at pH 8.0 and 30°C [Ruminococcus] torques
0.034
-
 7-oxo-lithocholic acid mutant enzyme G57S/I163V/V207M, at pH 8.0 and 30°C [Ruminococcus] torques
0.035
-
 7-oxo-lithocholic acid mutant enzyme V207M, at pH 8.0 and 30°C [Ruminococcus] torques
0.04
-
 7-oxo-lithocholic acid mutant enzyme T189V/V207M, at pH 8.0 and 30°C [Ruminococcus] torques
0.047
-
 NADPH mutant enzyme T189V/V207M, at pH 8.0 and 30°C [Ruminococcus] torques
0.057
-
 7-oxo-lithocholic acid mutant enzyme K44E/T189I, at pH 8.0 and 30°C [Ruminococcus] torques
0.19
-
 7-oxo-lithocholic acid mutant enzyme I112V/T189I/N240D, at pH 8.0 and 30°C [Ruminococcus] torques

Organism

Organism UniProt Comment Textmining
[Ruminococcus] torques
-
-
-
[Ruminococcus] torques ATCC 35915
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7-oxo-lithocholic acid + NADPH + H+
-
 [Ruminococcus] torques ursodeoxycholic acid + NADP+
-
 ?
7-oxo-lithocholic acid + NADPH + H+
-
 [Ruminococcus] torques ATCC 35915 ursodeoxycholic acid + NADP+
-
 ?

Synonyms

Synonyms Comment Organism
7beta-HSDH
-
 [Ruminococcus] torques

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
43
-
 melting temperature [Ruminococcus] torques

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.3
-
 7-oxo-lithocholic acid mutant enzyme I112V/T189I/N240D, at pH 8.0 and 30°C [Ruminococcus] torques
4.2
-
 7-oxo-lithocholic acid wild type enzyme, at pH 8.0 and 30°C [Ruminococcus] torques
4.3
-
 NADPH wild type enzyme, at pH 8.0 and 30°C [Ruminococcus] torques
7.8
-
 7-oxo-lithocholic acid mutant enzyme K44E/T189I, at pH 8.0 and 30°C [Ruminococcus] torques
13.4
-
 7-oxo-lithocholic acid mutant enzyme N240I, at pH 8.0 and 30°C [Ruminococcus] torques
17.3
-
 7-oxo-lithocholic acid mutant enzyme V207M, at pH 8.0 and 30°C [Ruminococcus] torques
17.5
-
 7-oxo-lithocholic acid mutant enzyme I28V/V38A/I76V/V207M, at pH 8.0 and 30°C [Ruminococcus] torques
20.1
-
 7-oxo-lithocholic acid mutant enzyme T189V, at pH 8.0 and 30°C [Ruminococcus] torques
21.4
-
 7-oxo-lithocholic acid mutant enzyme I112V/N240D, at pH 8.0 and 30°C [Ruminococcus] torques
23
-
 7-oxo-lithocholic acid mutant enzyme T189V/V207M, at pH 8.0 and 30°C [Ruminococcus] torques
25.1
-
 NADPH mutant enzyme T189V/V207M, at pH 8.0 and 30°C [Ruminococcus] torques
31
-
 7-oxo-lithocholic acid mutant enzyme G57S/I163V/V207M, at pH 8.0 and 30°C [Ruminococcus] torques

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 [Ruminococcus] torques

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 [Ruminococcus] torques

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
17
-
 7-oxo-lithocholic acid mutant enzyme I112V/T189I/N240D, at pH 8.0 and 30°C [Ruminococcus] torques
140
-
 7-oxo-lithocholic acid mutant enzyme K44E/T189I, at pH 8.0 and 30°C [Ruminococcus] torques
180
-
 7-oxo-lithocholic acid wild type enzyme, at pH 8.0 and 30°C [Ruminococcus] torques
480
-
 7-oxo-lithocholic acid mutant enzyme V207M, at pH 8.0 and 30°C [Ruminococcus] torques
530
-
 NADPH mutant enzyme T189V/V207M, at pH 8.0 and 30°C [Ruminococcus] torques
550
-
 NADPH wild type enzyme, at pH 8.0 and 30°C [Ruminococcus] torques
580
-
 7-oxo-lithocholic acid mutant enzyme N240I, at pH 8.0 and 30°C [Ruminococcus] torques
580
-
 7-oxo-lithocholic acid mutant enzyme T189V/V207M, at pH 8.0 and 30°C [Ruminococcus] torques
640
-
 7-oxo-lithocholic acid mutant enzyme T189V, at pH 8.0 and 30°C [Ruminococcus] torques
760
-
 7-oxo-lithocholic acid mutant enzyme I112V/N240D, at pH 8.0 and 30°C [Ruminococcus] torques
830
-
 7-oxo-lithocholic acid mutant enzyme I28V/V38A/I76V/V207M, at pH 8.0 and 30°C [Ruminococcus] torques
910
-
 7-oxo-lithocholic acid mutant enzyme G57S/I163V/V207M, at pH 8.0 and 30°C [Ruminococcus] torques