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Literature summary for 1.1.1.201 extracted from

  • Edenharder, R.; Pfutzner, A.; Hammann, R.
    NADP-dependent 3beta-, 7alpha- and 7beta-hydroxysteroid dehydrogenase activities from a lecithinase-lipase-negative Clostridium species 25.11.c (1989), Biochim. Biophys. Acta, 1002, 37-44.
    View publication on PubMed
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General Stability

General Stability Organism
heat-sensitive Clostridium sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
115000
-
 gel filtration Clostridium sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+ Clostridium sp. activity only in the reductive reaction 3alpha-hydroxy-7-oxo-5beta-cholanoic acid + NADPH
-
 ir
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+ Clostridium sp. 25.11.c activity only in the reductive reaction 3alpha-hydroxy-7-oxo-5beta-cholanoic acid + NADPH
-
 ir

Organism

Organism UniProt Comment Textmining
Clostridium sp.
-
-
-
Clostridium sp. 25.11.c
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Clostridium sp.
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+ activity only in the reductive reaction Clostridium sp. 3alpha-hydroxy-7-oxo-5beta-cholanoic acid + NADPH
-
 ir
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+ activity only in the reductive reaction Clostridium sp. 25.11.c 3alpha-hydroxy-7-oxo-5beta-cholanoic acid + NADPH
-
 ir

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.6
-
-
 Clostridium sp.

Cofactor

Cofactor Comment Organism Structure
NADP+ no activity with NAD+ Clostridium sp.