General Stability | Organism |
---|---|
presence of reducing agents protects against thermal denaturation | Blautia producta |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.055 | - |
3alpha-hydroxy-7-keto-5beta-cholanoic acid | - |
Blautia producta | |
0.07 | - |
3,12-diketocholanoic acid | - |
Blautia producta | |
0.087 | - |
3,7-Diketo-5beta-cholanoic acid | - |
Blautia producta | |
0.123 | - |
3,7,12-Triketo-5beta-cholanoic acid | - |
Blautia producta | |
0.124 | - |
3alpha-Hydroxy-7,12-diketo-5beta-cholanoic acid | - |
Blautia producta | |
0.142 | - |
3alpha,12alpha-dihydroxy-7-keto-5beta-cholanoic acid | - |
Blautia producta | |
0.145 | - |
3,7,12-triketo-5beta-cholanoyl taurine | - |
Blautia producta |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
intracellular | - |
Blautia producta | 5622 | - |
membrane | - |
Blautia producta | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
82000 | - |
gel filtration | Blautia producta |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,7,12-triketo-5beta-cholanoic acid + NADPH | Blautia producta | - |
7beta-hydroxy-3,12-diketo-5beta-cholanoic acid + NADP+ | - |
? | |
3,7,12-triketo-5beta-cholanoyl taurine + NADPH | Blautia producta | - |
7beta-hydroxy-3,12-diketo-5beta-cholanoyl taurine + NADP+ | - |
? | |
3,7-diketo-5beta-cholanoic acid + NADPH | Blautia producta | - |
7beta-hydroxy-3-keto-5beta-cholanoic acid + NADP+ | - |
? | |
3,7-diketo-5beta-cholanoic acid + NADPH | Blautia producta K2/25-1 | - |
7beta-hydroxy-3-keto-5beta-cholanoic acid + NADP+ | - |
? | |
3alpha,12alpha-dihydroxy-7-keto-5beta-cholanoic acid + NADPH | Blautia producta | - |
3alpha,7beta,12alpha-trihydroxy-5beta-cholanoic acid + NADP+ | - |
? | |
3alpha,12alpha-dihydroxy-7-keto-5beta-cholanoic acid + NADPH | Blautia producta K2/25-1 | - |
3alpha,7beta,12alpha-trihydroxy-5beta-cholanoic acid + NADP+ | - |
? | |
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+ | Blautia producta | ursodeoxycholic acid | 3alpha-hydroxy-7-oxo-5beta-cholanoic acid + NADPH | - |
? | |
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+ | Blautia producta K2/25-1 | ursodeoxycholic acid | 3alpha-hydroxy-7-oxo-5beta-cholanoic acid + NADPH | - |
? | |
3alpha-hydroxy-7,12-diketo-5beta-cholanoic acid + NADPH | Blautia producta | - |
3alpha,7beta-dihydroxy-12-keto-5beta-cholanoic acid + NADP+ | - |
? | |
3alpha-hydroxy-7,12-diketo-5beta-cholanoic acid + NADPH | Blautia producta K2/25-1 | - |
3alpha,7beta-dihydroxy-12-keto-5beta-cholanoic acid + NADP+ | - |
? | |
7,12-diketo-5beta-cholanoic acid + NADPH | Blautia producta | - |
7beta-hydroxy-12-keto-5beta-cholanoic acid + NADP+ | - |
? | |
7,12-diketo-5beta-cholanoic acid + NADPH | Blautia producta K2/25-1 | - |
7beta-hydroxy-12-keto-5beta-cholanoic acid + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Blautia producta | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Blautia producta | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,12-diketocholanoic acid + NADP+ | - |
Blautia producta | ? | - |
r | |
3,7,12-triketo-5beta-cholanoic acid + NADPH | - |
Blautia producta | 7beta-hydroxy-3,12-diketo-5beta-cholanoic acid + NADP+ | - |
? | |
3,7,12-triketo-5beta-cholanoyl taurine + NADPH | - |
Blautia producta | 7beta-hydroxy-3,12-diketo-5beta-cholanoyl taurine + NADP+ | - |
? | |
3,7-diketo-5beta-cholanoic acid + NADPH | - |
Blautia producta | 7beta-hydroxy-3-keto-5beta-cholanoic acid + NADP+ | - |
? | |
3,7-diketo-5beta-cholanoic acid + NADPH | - |
Blautia producta K2/25-1 | 7beta-hydroxy-3-keto-5beta-cholanoic acid + NADP+ | - |
? | |
3alpha,12alpha-dihydroxy-7-keto-5beta-cholanoic acid + NADPH | - |
Blautia producta | 3alpha,7beta,12alpha-trihydroxy-5beta-cholanoic acid + NADP+ | - |
? | |
3alpha,12alpha-dihydroxy-7-keto-5beta-cholanoic acid + NADPH | - |
Blautia producta K2/25-1 | 3alpha,7beta,12alpha-trihydroxy-5beta-cholanoic acid + NADP+ | - |
? | |
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+ | ursodeoxycholic acid | Blautia producta | 3alpha-hydroxy-7-oxo-5beta-cholanoic acid + NADPH | - |
? | |
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+ | ursodeoxycholic acid | Blautia producta K2/25-1 | 3alpha-hydroxy-7-oxo-5beta-cholanoic acid + NADPH | - |
? | |
3alpha-hydroxy-7,12-diketo-5beta-cholanoic acid + NADPH | - |
Blautia producta | 3alpha,7beta-dihydroxy-12-keto-5beta-cholanoic acid + NADP+ | - |
? | |
3alpha-hydroxy-7,12-diketo-5beta-cholanoic acid + NADPH | - |
Blautia producta K2/25-1 | 3alpha,7beta-dihydroxy-12-keto-5beta-cholanoic acid + NADP+ | - |
? | |
7,12-diketo-5beta-cholanoic acid + NADPH | - |
Blautia producta | 7beta-hydroxy-12-keto-5beta-cholanoic acid + NADP+ | - |
? | |
7,12-diketo-5beta-cholanoic acid + NADPH | - |
Blautia producta K2/25-1 | 7beta-hydroxy-12-keto-5beta-cholanoic acid + NADP+ | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
50% loss of activity within 60 min | Blautia producta |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10 | - |
- |
Blautia producta |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | no activity with NAD+ | Blautia producta |