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Literature summary for 1.1.1.201 extracted from

  • Hirano, S.; Masuda, N.
    Characterization of NADP-dependent 7beta-hydroxysteroid dehydrogenases from Peptostreptococcus productus and Eubacterium aerofaciens (1982), Appl. Environ. Microbiol., 43, 1057-1063.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
iodoacetate complete inhibition at 0.05 mM Blautia producta
p-chloromercuribenzoic acid complete inhibition at 0.001 mM Blautia producta

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.022
-
3alpha,7beta-dihydroxy-5beta-cholanoic acid
-
Blautia producta
0.1
-
NADP+
-
Blautia producta
0.108
-
3alpha,7beta-dihydroxy-5beta-cholanoic acid
-
Collinsella aerofaciens
0.238
-
3alpha,7beta-dihydroxy-5beta-cholanoyl glycine
-
Blautia producta
0.286
-
3alpha,7beta-dihydroxy-5beta-cholanoyl taurine
-
Blautia producta
0.4
-
NADP+
-
Collinsella aerofaciens
0.909
-
3alpha,7beta-dihydroxy-5beta-cholanoyl glycine
-
Collinsella aerofaciens
1.639
-
3alpha,7beta-dihydroxy-5beta-cholanoyl taurine
-
Collinsella aerofaciens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
gel filtration Collinsella aerofaciens
53000
-
gel filtration Blautia producta

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+ Collinsella aerofaciens ursodeoxycholic acid 3alpha-hydroxy-7-oxo-5beta-cholanoic acid + NADPH
-
?
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+ Blautia producta ursodeoxycholic acid 3alpha-hydroxy-7-oxo-5beta-cholanoic acid + NADPH
-
?
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+ Collinsella aerofaciens only active on free and conjugated bile salts 3alpha-hydroxy-7-oxo-5beta-cholanoic acid + NADPH
-
?
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+ Blautia producta only active on free and conjugated bile salts 3alpha-hydroxy-7-oxo-5beta-cholanoic acid + NADPH
-
?
3alpha,7beta-dihydroxy-5beta-cholanoyl glycine + NADP+ Collinsella aerofaciens
-
3alpha-hydroxy-7-keto-5beta-cholanoyl glycine + NADPH
-
?
3alpha,7beta-dihydroxy-5beta-cholanoyl glycine + NADP+ Blautia producta
-
3alpha-hydroxy-7-keto-5beta-cholanoyl glycine + NADPH
-
?
3alpha,7beta-dihydroxy-5beta-cholanoyl taurine + NADP+ Collinsella aerofaciens
-
3alpha-hydroxy-7-keto-5beta-cholanoyl taurine + NADPH
-
?
3alpha,7beta-dihydroxy-5beta-cholanoyl taurine + NADP+ Blautia producta
-
3alpha-hydroxy-7-keto-5beta-cholanoyl taurine + NADPH
-
?

Organism

Organism UniProt Comment Textmining
Blautia producta
-
-
-
Collinsella aerofaciens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography techniques Collinsella aerofaciens
to homogeneity, chromatography techniques Blautia producta

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
Collinsella aerofaciens
-
cell culture
-
Blautia producta
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.1
-
crude cell extract Collinsella aerofaciens
0.17
-
-
Collinsella aerofaciens
1.06
-
-
Blautia producta

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+ ursodeoxycholic acid Collinsella aerofaciens 3alpha-hydroxy-7-oxo-5beta-cholanoic acid + NADPH
-
?
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+ ursodeoxycholic acid Blautia producta 3alpha-hydroxy-7-oxo-5beta-cholanoic acid + NADPH
-
?
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+ only active on free and conjugated bile salts Collinsella aerofaciens 3alpha-hydroxy-7-oxo-5beta-cholanoic acid + NADPH
-
?
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+ only active on free and conjugated bile salts Blautia producta 3alpha-hydroxy-7-oxo-5beta-cholanoic acid + NADPH
-
?
3alpha,7beta-dihydroxy-5beta-cholanoyl glycine + NADP+
-
Collinsella aerofaciens 3alpha-hydroxy-7-keto-5beta-cholanoyl glycine + NADPH
-
?
3alpha,7beta-dihydroxy-5beta-cholanoyl glycine + NADP+
-
Blautia producta 3alpha-hydroxy-7-keto-5beta-cholanoyl glycine + NADPH
-
?
3alpha,7beta-dihydroxy-5beta-cholanoyl taurine + NADP+
-
Collinsella aerofaciens 3alpha-hydroxy-7-keto-5beta-cholanoyl taurine + NADPH
-
?
3alpha,7beta-dihydroxy-5beta-cholanoyl taurine + NADP+
-
Blautia producta 3alpha-hydroxy-7-keto-5beta-cholanoyl taurine + NADPH
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
50% loss of activity within 30 min Blautia producta
50
-
90% loss of activity within 3 min Blautia producta

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.1 11
-
Collinsella aerofaciens
9.5 10.5
-
Blautia producta

Cofactor

Cofactor Comment Organism Structure
NADP+ no activity with NAD+ Collinsella aerofaciens
NADP+ no activity with NAD+ Blautia producta