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Literature summary for 1.1.1.20 extracted from

  • Hayashi, S.; Watanabe, M.; Kimura, A.
    Enzymatic determination of free glucoronic acid with glucuronolactone reductase. I. isolation and purification of glucoronolactone reductase from rat kidney (1984), J. Biochem., 95, 223-232.
    View publication on PubMed

General Stability

General Stability Organism
freezing and thawing: 3 times causes 25% decrease of activity, 5 times causes 40% decrease of activity Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
Barbital
-
Rattus norvegicus
barbiturates
-
Rattus norvegicus
dithiothreitol
-
Rattus norvegicus
Hg2+
-
Rattus norvegicus
iodoacetic acid
-
Rattus norvegicus
NaF
-
Rattus norvegicus
Phenobarbital
-
Rattus norvegicus
Zn2+
-
Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.045
-
NADPH with D-glucuronic acid Rattus norvegicus
4
-
D-galacturonic acid
-
Rattus norvegicus
6
-
D-glucuronic acid L-iduronic acid Rattus norvegicus
9
-
D-glucuronolactone
-
Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Rattus norvegicus 5737
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
1 * 40000, SDS-PAGE Rattus norvegicus
41000
-
gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-gulono-1,4-lactone + NADP+ Rattus norvegicus
-
D-glucurono-3,6-lactone + NADPH
-
?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
13450 U/min * mg, 1 unit is defined as change of absorbance of 0.01/min at 340 nm Rattus norvegicus

Storage Stability

Storage Stability Organism
-80°C, 10 days, 30% loss of activity Rattus norvegicus
0°C, 2 months, less than 10% loss of activity Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-galacturonic acid + NADPH
-
Rattus norvegicus ?
-
?
D-glucuronic acid + NADPH
-
Rattus norvegicus L-gulonic acid + NADP+
-
?
D-glucurono-3,6-lactone + NADPH oxidation activity is very low Rattus norvegicus D-gulono-1,4-lactone + NADP+
-
r
D-iduronic acid + NADPH
-
Rattus norvegicus ?
-
?
erythrose + NADPH
-
Rattus norvegicus glycerol + NADP+
-
?
glyceraldehyde + NADPH
-
Rattus norvegicus ?
-
?
L-gulono-1,4-lactone + NADP+
-
Rattus norvegicus D-glucurono-3,6-lactone + NADPH
-
?

Subunits

Subunits Comment Organism
monomer 1 * 40000, SDS-PAGE Rattus norvegicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
30 min, 20% loss of activity Rattus norvegicus
40
-
60 min, 30% loss of activity Rattus norvegicus
40
-
180 min, 50% loss of activity Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 7.5 DL-glyceraldehyde Rattus norvegicus
6 7 D-glucuronic acid Rattus norvegicus
9.5
-
broad, oxidation of glycerol and L-gulonolactone Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
NADH NADPH is 145-times as effective as NADH Rattus norvegicus
NADP+
-
Rattus norvegicus
NADPH NADPH is 145-times as effective as NADH Rattus norvegicus