Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 1 mM, complete loss of activity | Mycobacterium sp. | |
iodoacetic acid | 1 mM, 72.6% residual activity | Mycobacterium sp. | |
NaN3 | 1 mM, complete loss of activity | Mycobacterium sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10.27 | - |
3-methyl-1,5-pentanediol | pH 7.0. 30°C | Mycobacterium sp. | |
23.38 | - |
1,5-pentanediol | pH 7.0. 30°C | Mycobacterium sp. | |
26.88 | - |
NADP+ | pH 7.0. 30°C | Mycobacterium sp. | |
43.75 | - |
butan-1-ol | pH 7.0. 30°C | Mycobacterium sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Mycobacterium sp. | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | 1 mM, 467% of initial activity | Mycobacterium sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
- |
Mycobacterium sp. |
68000 | - |
gel filtration | Mycobacterium sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium sp. | A0A125SUY5 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,5-pentanediol + NADP+ | - |
Mycobacterium sp. | ? + NADPH + H+ | - |
? | |
3-methyl-1,5-pentanediol + NADP+ | - |
Mycobacterium sp. | ? | - |
? | |
butan-1-ol + NADP+ | - |
Mycobacterium sp. | butanal + NADPH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 38000, SDS-PAGE, 2 * 36907, calculated | Mycobacterium sp. |
Synonyms | Comment | Organism |
---|---|---|
Mpd1 | - |
Mycobacterium sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Mycobacterium sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
1 h, complete inactivation above 45°C | Mycobacterium sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.31 | - |
1,5-pentanediol | pH 7.0. 30°C | Mycobacterium sp. | |
7.02 | - |
butan-1-ol | pH 7.0. 30°C | Mycobacterium sp. | |
7.84 | - |
3-methyl-1,5-pentanediol | pH 7.0. 30°C | Mycobacterium sp. | |
13.41 | - |
NADP+ | pH 7.0. 30°C | Mycobacterium sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 7.5 | - |
Mycobacterium sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no cofoactor: NADH | Mycobacterium sp. | |
NADPH | - |
Mycobacterium sp. |
General Information | Comment | Organism |
---|---|---|
physiological function | bacterium Mycobacterium sp. B-009, is able to grow on racemic 1,2-propanediol. The strain oxidizes 3-methyl-1,5-pentanediol to 5-hydroxy-3-methyl-pentanoic acid via the S-form of 5-hydroxy-3-methyl-pentanoic acid. The enzyme is an enantioselective 3-methyl-1,5-pentanediol dehydrogenase | Mycobacterium sp. |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
160 | - |
butan-1-ol | pH 7.0. 30°C | Mycobacterium sp. | |
230 | - |
1,5-pentanediol | pH 7.0. 30°C | Mycobacterium sp. | |
500 | - |
NADP+ | pH 7.0. 30°C | Mycobacterium sp. | |
760 | - |
3-methyl-1,5-pentanediol | pH 7.0. 30°C | Mycobacterium sp. |