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Literature summary for 1.1.1.2 extracted from

  • Basen, M.; Schut, G.J.; Nguyen, D.M.; Lipscomb, G.L.; Benn, R.A.; Prybol, C.J.; Vaccaro, B.J.; Poole, F.L.; Kelly, R.M.; Adams, M.W.
    Single gene insertion drives bioalcohol production by a thermophilic archaeon (2014), Proc. Natl. Acad. Sci. USA, 111, 17618-17623.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
synthesis synthetic pathway for bioalcohol production at 70°C by insertion of the gene for bacterial alcohol dehydrogenase AdhA into the archaeon Pyrococcus furiosus. The engineered strain converts glucose to ethanol via acetate and acetaldehyde, catalyzed by the host-encoded aldehyde ferredoxin oxidoreductase AOR and heterologously expressed AdhA, in an energy-conserving, redox-balanced pathway. The AOR/AdhA pathway also converts exogenously added aliphatic and aromatic carboxylic acids to the corresponding alcohol using glucose, pyruvate, and/or hydrogen as the source of reductant. By heterologous coexpression of a membrane-bound carbon monoxide dehydrogenase, CO is used as a reductant for converting carboxylic acids to alcoholsThe AOR/AdhA pathway is a potentially game-changing strategy for syngas fermentation, especially in combination with carbon chain elongation pathways Thermoanaerobacter sp.

Organism

Organism UniProt Comment Textmining
Thermoanaerobacter sp. B0K3U7
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Synonyms

Synonyms Comment Organism
AdhA
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Thermoanaerobacter sp.
Teth514_0564
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Thermoanaerobacter sp.