Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Thermococcus sibiricus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 5 mM, 80% of initial activity | Thermococcus sibiricus | |
Fe2+ | 10 mM, 74% of initial activity | Thermococcus sibiricus | |
Mg2+ | 10 mM, 78% of initial activity | Thermococcus sibiricus | |
Zn2+ | 10 mM, 79% of initial activity | Thermococcus sibiricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
NADPH | pH 7.5, 60°C | Thermococcus sibiricus | |
0.022 | - |
NADP+ | pH 10.5, 60°C | Thermococcus sibiricus | |
17.75 | - |
Pyruvaldehyde | pH 7.5, 60°C | Thermococcus sibiricus | |
54.4 | - |
D-xylose | pH 10.5, 60°C | Thermococcus sibiricus | |
168 | - |
2-propanol | pH 10.5, 60°C | Thermococcus sibiricus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | 600 mM, 147% of initial activity | Thermococcus sibiricus | |
NaCl | 400 mM, 206% of initial activity | Thermococcus sibiricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermococcus sibiricus | C6A190 | - |
- |
Thermococcus sibiricus DSM 12597 | C6A190 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-2-butanol + NADP+ | 196% of the activity with 2-propanol, 2fold preference for (S)-2-butanol over (RS)-2-butanol | Thermococcus sibiricus | butanone + NADPH + H+ | - |
? | |
2-pentanol + NADP+ | 67% of the activity with 2-propanol | Thermococcus sibiricus | pentan-2-one + NADPH + H+ | - |
? | |
2-propanol + NADP+ | - |
Thermococcus sibiricus | acetone + NADPH + H+ | - |
? | |
2-propanol + NADP+ | - |
Thermococcus sibiricus DSM 12597 | acetone + NADPH + H+ | - |
? | |
benzyl alcohol + NADP+ | 180% of the activity with 2-propanol | Thermococcus sibiricus | benzaldehyde + NADPH + H+ | - |
? | |
benzyl alcohol + NADP+ | 180% of the activity with 2-propanol | Thermococcus sibiricus DSM 12597 | benzaldehyde + NADPH + H+ | - |
? | |
cellobiose + NADP+ | 71% of the activity with 2-propanol | Thermococcus sibiricus | ? + NADPH + H+ | - |
? | |
cellobiose + NADP+ | 71% of the activity with 2-propanol | Thermococcus sibiricus DSM 12597 | ? + NADPH + H+ | - |
? | |
D-arabinose + NADP+ | 200% of the activity with 2-propanol. D-arabinose is preferred over L-arabinose | Thermococcus sibiricus | D-arabitol + NADPH + H+ | - |
? | |
D-glucose + NADP+ | 146% of the activity with 2-propanol | Thermococcus sibiricus | ? + NADPH + H+ | - |
? | |
D-glucose + NADP+ | 146% of the activity with 2-propanol | Thermococcus sibiricus DSM 12597 | ? + NADPH + H+ | - |
? | |
D-xylose + NADP+ | 246% of the activity with 2-propanol | Thermococcus sibiricus | ? + NADPH + H+ | - |
? | |
dimethylglyoxal + NADPH + H+ | 270% of the activity with pyruvaldehyde | Thermococcus sibiricus | ? + NADP+ | - |
? | |
pyruvaldehyde + NADPH + H+ | - |
Thermococcus sibiricus | lactaldehyde + NADP+ | - |
? | |
pyruvaldehyde + NADPH + H+ | - |
Thermococcus sibiricus DSM 12597 | lactaldehyde + NADP+ | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
1.5 h, no loss of activity | Thermococcus sibiricus |
80 | - |
1.5 h, no loss of activity | Thermococcus sibiricus |
90 | - |
half-life 2 h | Thermococcus sibiricus |
100 | - |
half-life 1 h | Thermococcus sibiricus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.45 | - |
NADP+ | pH 10.5, 60°C | Thermococcus sibiricus | |
0.53 | - |
2-propanol | pH 10.5, 60°C | Thermococcus sibiricus | |
0.7 | - |
D-xylose | pH 10.5, 60°C | Thermococcus sibiricus | |
1.51 | - |
NADPH | pH 7.5, 60°C | Thermococcus sibiricus | |
2.04 | - |
Pyruvaldehyde | pH 7.5, 60°C | Thermococcus sibiricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at, reduction of ketones | Thermococcus sibiricus |
10.5 | - |
assay at, oxidation of alcohols | Thermococcus sibiricus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Thermococcus sibiricus | |
NADPH | - |
Thermococcus sibiricus |