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Literature summary for 1.1.1.2 extracted from

  • Stekhanova, T.N.; Mardanov, A.V.; Bezsudnova, E.Y.; Gumerov, V.M.; Ravin, N.V.; Skryabin, K.G.; Popov, V.O.
    Characterization of a thermostable short-chain alcohol dehydrogenase from the hyperthermophilic archaeon Thermococcus sibiricus (2010), Appl. Environ. Microbiol., 76, 4096-4098.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.2

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Thermococcus sibiricus

Inhibitors

Inhibitors Comment Organism Structure
EDTA 5 mM, 80% of initial activity Thermococcus sibiricus
Fe2+ 10 mM, 74% of initial activity Thermococcus sibiricus
Mg2+ 10 mM, 78% of initial activity Thermococcus sibiricus
Zn2+ 10 mM, 79% of initial activity Thermococcus sibiricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
 NADPH pH 7.5, 60°C Thermococcus sibiricus
0.022
-
 NADP+ pH 10.5, 60°C Thermococcus sibiricus
17.75
-
 Pyruvaldehyde pH 7.5, 60°C Thermococcus sibiricus
54.4
-
 D-xylose pH 10.5, 60°C Thermococcus sibiricus
168
-
 2-propanol pH 10.5, 60°C Thermococcus sibiricus

Metals/Ions

Metals/Ions Comment Organism Structure
KCl 600 mM, 147% of initial activity Thermococcus sibiricus
NaCl 400 mM, 206% of initial activity Thermococcus sibiricus

Organism

Organism UniProt Comment Textmining
Thermococcus sibiricus C6A190
-
-
Thermococcus sibiricus DSM 12597 C6A190
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-2-butanol + NADP+ 196% of the activity with 2-propanol, 2fold preference for (S)-2-butanol over (RS)-2-butanol Thermococcus sibiricus butanone + NADPH + H+
-
 ?
2-pentanol + NADP+ 67% of the activity with 2-propanol Thermococcus sibiricus pentan-2-one + NADPH + H+
-
 ?
2-propanol + NADP+
-
 Thermococcus sibiricus acetone + NADPH + H+
-
 ?
2-propanol + NADP+
-
 Thermococcus sibiricus DSM 12597 acetone + NADPH + H+
-
 ?
benzyl alcohol + NADP+ 180% of the activity with 2-propanol Thermococcus sibiricus benzaldehyde + NADPH + H+
-
 ?
benzyl alcohol + NADP+ 180% of the activity with 2-propanol Thermococcus sibiricus DSM 12597 benzaldehyde + NADPH + H+
-
 ?
cellobiose + NADP+ 71% of the activity with 2-propanol Thermococcus sibiricus ? + NADPH + H+
-
 ?
cellobiose + NADP+ 71% of the activity with 2-propanol Thermococcus sibiricus DSM 12597 ? + NADPH + H+
-
 ?
D-arabinose + NADP+ 200% of the activity with 2-propanol. D-arabinose is preferred over L-arabinose Thermococcus sibiricus D-arabitol + NADPH + H+
-
 ?
D-glucose + NADP+ 146% of the activity with 2-propanol Thermococcus sibiricus ? + NADPH + H+
-
 ?
D-glucose + NADP+ 146% of the activity with 2-propanol Thermococcus sibiricus DSM 12597 ? + NADPH + H+
-
 ?
D-xylose + NADP+ 246% of the activity with 2-propanol Thermococcus sibiricus ? + NADPH + H+
-
 ?
dimethylglyoxal + NADPH + H+ 270% of the activity with pyruvaldehyde Thermococcus sibiricus ? + NADP+
-
 ?
pyruvaldehyde + NADPH + H+
-
 Thermococcus sibiricus lactaldehyde + NADP+
-
 ?
pyruvaldehyde + NADPH + H+
-
 Thermococcus sibiricus DSM 12597 lactaldehyde + NADP+
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 1.5 h, no loss of activity Thermococcus sibiricus
80
-
 1.5 h, no loss of activity Thermococcus sibiricus
90
-
 half-life 2 h Thermococcus sibiricus
100
-
 half-life 1 h Thermococcus sibiricus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.45
-
 NADP+ pH 10.5, 60°C Thermococcus sibiricus
0.53
-
 2-propanol pH 10.5, 60°C Thermococcus sibiricus
0.7
-
 D-xylose pH 10.5, 60°C Thermococcus sibiricus
1.51
-
 NADPH pH 7.5, 60°C Thermococcus sibiricus
2.04
-
 Pyruvaldehyde pH 7.5, 60°C Thermococcus sibiricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at, reduction of ketones Thermococcus sibiricus
10.5
-
 assay at, oxidation of alcohols Thermococcus sibiricus

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Thermococcus sibiricus
NADPH
-
 Thermococcus sibiricus