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Literature summary for 1.1.1.2 extracted from

  • Liu, Y.; Jing, K.; Jiang, X.; Lin, J.; Wang, F.; Cen, P.
    Asymmetric reduction of ethyl 4-chloro-3-oxobutanoate to ethyl (R)-4-chloro-3-hydroxybutanoate with two co-existing, recombinant Escherichia coli strains (2005), Biotechnol. Lett., 27, 119-125.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli M15, plasmid pQE30-alr0307, cultivation in LB medium, ampicillin, kanamycin, 37°C Sporidiobolus salmonicolor

Organism

Organism UniProt Comment Textmining
Sporidiobolus salmonicolor
-
ZJU0307
-
Sporidiobolus salmonicolor ZJU0307
-
ZJU0307
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.19
-
activity of ALR in Escherichia coli M15, pH 6.5, assay mixture including potassium phosphate buffer, NADPH and p-nitrobenzaldehyde Sporidiobolus salmonicolor
0.42
-
activiy of ALR in Sporobolomyces salmonicolor, pH 6.5, assay mixture including potassium phosphate buffer, NADPH and p-nitrobenzaldehyde Sporidiobolus salmonicolor
6.57
-
activiy of ALR in Eschericher coli M15 with the plasmid pQE30-alr0307, pH 6.5, assay mixture including potassium phosphate buffer, NADPH and p-nitrobenzaldehyde Sporidiobolus salmonicolor

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ethyl-4-chloro-3-oxobutanoate + NADPH + H+
-
Sporidiobolus salmonicolor ethyl-4-chloro-3-hydroxybutanoate + NADP+
-
?
ethyl-4-chloro-3-oxobutanoate + NADPH + H+
-
Sporidiobolus salmonicolor ZJU0307 ethyl-4-chloro-3-hydroxybutanoate + NADP+
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay temperature Sporidiobolus salmonicolor

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Sporidiobolus salmonicolor