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Literature summary for 1.1.1.2 extracted from

  • Munir, I.; Nakazawa, M.; Harano, K.; Yamaji, R.; Inui, H.; Miyatake, K.; Nakano, Y.
    Occurrence of a novel NADP(+)-linked alcohol dehydrogenase in Euglena gracilis (2002), Comp. Biochem. Physiol. B, 132, 535-540.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.79
-
1-Hexanol pH 8.8, 30°C Euglena gracilis
46.7
-
NADP+ pH 8.8, 30°C Euglena gracilis

Organism

Organism UniProt Comment Textmining
Euglena gracilis
-
growth on 1-hexanol, induction of enzyme
-

Storage Stability

Storage Stability Organism
4°C, extremely unstable, 20% ethylene glycol stabilizes Euglena gracilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-butanol + NADP+ 25% of activity with 1-hexanol Euglena gracilis butanal + NADPH
-
r
1-hexanol + NADP+
-
Euglena gracilis hexanal + NADPH
-
r
1-octanol + NADP+ 31% of activity with 1-hexanol Euglena gracilis octanal + NADPH
-
r
1-pentanol + NADP+ 58% of activity with 1-hexanol Euglena gracilis pentanal + NADPH
-
r

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
10 min, 67% residual activity Euglena gracilis
50
-
10 min, complete loss of activity Euglena gracilis

pH Range

pH Minimum pH Maximum Comment Organism
8.8 9 oxidation reaction Euglena gracilis