Literature summary for 1.1.1.2 extracted from

Peralba, J.M.; Cederlund, E.; Crosas, B.; Moreno, A.; Julia, P.; Martinez, S.E.; Persson, B.; Farr s, J.; Pares, X.; Jornvall, H.
Structural and enzymatic properties of a gastric NADP(H)-dependent and retinal-active alcohol dehydrogenase (1999), J. Biol. Chem., 274, 26021-26026.

Cloned(Commentary)

Cloned (Comment)	Organism
frog stomach ADH cDNA cloned	Pelophylax perezi

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism
0.002	-	m-nitrobenzaldehyde	-	Pelophylax perezi
0.008	-	all-trans-retinal	-	Pelophylax perezi
0.009	-	9-cis-retinal	-	Pelophylax perezi
0.01	-	octanal	-	Pelophylax perezi
0.012	-	NADPH	m-nitrobenzaldehyde as substrate	Pelophylax perezi
0.015	-	13-cis-retinal	-	Pelophylax perezi
0.019	-	9-cis-retinol	-	Pelophylax perezi
0.02	-	all-trans-retinol	-	Pelophylax perezi
0.03	-	NADP+	-	Pelophylax perezi
0.04	-	hexanal	-	Pelophylax perezi
0.06	-	trans-2-hexenal	-	Pelophylax perezi
0.06	-	NADP+	ethanol as substrate	Pelophylax perezi
0.07	-	pentanal	-	Pelophylax perezi
0.09	-	Octanol	-	Pelophylax perezi
0.11	-	Butanal	-	Pelophylax perezi
0.15	-	Octanol	-	Pelophylax perezi
0.2	-	NAD+	ethanol as substrate	Pelophylax perezi
0.22	-	trans-2-hexenol	-	Pelophylax perezi
0.22	-	Pentanol	at pH 10	Pelophylax perezi
0.35	-	Hexanol	-	Pelophylax perezi
0.52	-	NADH	m-nitrobenzaldehyde as substrate	Pelophylax perezi
0.8	-	NAD+	octanol as substrate	Pelophylax perezi
1.1	-	Pentanol	-	Pelophylax perezi
1.7	-	butanol	at pH 10	Pelophylax perezi
7	-	acetaldehyde	-	Pelophylax perezi
8.2	-	butanol	-	Pelophylax perezi
70	-	ethanol	-	Pelophylax perezi
170	-	2-butanol	-	Pelophylax perezi
600	-	ethanol	-	Pelophylax perezi

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
44000	-	2 * 44000, SDS-PAGE	Pelophylax perezi
80000	-	gel filtration	Pelophylax perezi

Organism

Organism	UniProt	Comment	Textmining
Clostridium beijerinckii	-	-	-
Entamoeba histolytica	-	-	-
Pelophylax perezi	-	frog	-
Thermoanaerobacter brockii	-	-	-
Thermoanaerobacter ethanolicus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Pelophylax perezi

Source Tissue

Source Tissue	Comment	Organism	Textmining
liver	-	Pelophylax perezi	-
stomach	-	Pelophylax perezi	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
(2E)-hex-2-enal + NADPH + H+	-	Pelophylax perezi	(2E)-2-hexen-1-ol + NADP+	-	r
1-octanol + NADP+	-	Pelophylax perezi	octanal + NADPH	-	r
1-pentanol + NADP+	-	Thermoanaerobacter ethanolicus	n-pentanal + NADPH	-	r
1-pentanol + NADP+	-	Pelophylax perezi	n-pentanal + NADPH	-	r
13-cis-retinal + NADPH	-	Pelophylax perezi	13-cis-retinol + NADP+	-	?
2-butanol + NADP+	-	Thermoanaerobacter ethanolicus	butan-2-one + NADPH	-	r
2-butanol + NADP+	-	Thermoanaerobacter brockii	butan-2-one + NADPH	-	r
2-butanol + NADP+	-	Pelophylax perezi	butan-2-one + NADPH	-	r
3-nitrobenzaldehyde + NADH + H+	-	Pelophylax perezi	3-nitrobenzyl alcohol + NAD+	-	r
9-cis-retinal + NADPH	-	Pelophylax perezi	9-cis-retinol + NADP+	-	r
9-cis-retinol + NADP+	-	Pelophylax perezi	9-cis-retinal + NADPH	-	r
acetaldehyde + NADPH + H+	-	Thermoanaerobacter ethanolicus	ethanol + NADP+	-	?
acetaldehyde + NADPH + H+	-	Pelophylax perezi	ethanol + NADP+	-	?
all-trans-retinal + NADPH + H+	-	Pelophylax perezi	all-trans-retinol + NADP+	-	r
all-trans-retinol + NADP+	-	Pelophylax perezi	all-trans-retinal + NADPH	-	r
ethanol + NAD+	-	Pelophylax perezi	acetaldehyde + NADH + H+	-	r
ethanol + NADP+	-	Clostridium beijerinckii	acetaldehyde + NADPH + H+	-	r
ethanol + NADP+	-	Thermoanaerobacter ethanolicus	acetaldehyde + NADPH + H+	-	r
ethanol + NADP+	-	Thermoanaerobacter brockii	acetaldehyde + NADPH + H+	-	r
ethanol + NADP+	-	Pelophylax perezi	acetaldehyde + NADPH + H+	-	r
hexanal + NADPH + H+	-	Pelophylax perezi	hexanol + NADP+	-	r
m-nitrobenzaldehyde + NADPH	-	Pelophylax perezi	m-nitrobenzylalcohol + NADP+	-	r
n-butanol + NADP+	-	Thermoanaerobacter ethanolicus	butyraldehyde + NADPH	-	r
n-butanol + NADP+	-	Pelophylax perezi	butyraldehyde + NADPH	-	r
n-butyraldehyde + NADPH	-	Thermoanaerobacter ethanolicus	n-butanol + NADP+	-	r
n-butyraldehyde + NADPH	-	Pelophylax perezi	n-butanol + NADP+	-	r
n-hexanol + NADP+	-	Pelophylax perezi	n-hexanal + NADPH	-	r
n-octanal + NADPH + H+	-	Pelophylax perezi	n-octanol + NADP+	-	r
octanol + NAD+	-	Pelophylax perezi	octanal + NADH + H+	-	r
octanol + NADP+	-	Pelophylax perezi	octanal + NADPH + H+	-	r
pentanal + NADPH	-	Pelophylax perezi	pentanol + NADP+	-	r
trans-2-hexenol + NADP+	-	Pelophylax perezi	trans-2-hexenal + NADPH	-	r

Subunits

Subunits	Comment	Organism
dimer	2 * 44000, SDS-PAGE	Pelophylax perezi