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Literature summary for 1.1.1.195 extracted from

  • Ragamustari, S.; Shiraiwa, N.; Hattori, T.; Nakatsubo, T.; Suzuki, S.; Umezawa, T.
    Characterization of three cinnamyl alcohol dehydrogenases from Carthamus tinctorius (2013), Plant Biotechnol., 30, 315-326.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
gene ctcad1, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, quantitative realtime PCR enzyme expression analysis Carthamus tinctorius
gene ctcad2, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, quantitative realtime PCR enzyme expression analysis Carthamus tinctorius
quantitative realtime PCR enzyme expression analysis during seed development, overview. CAD2 shows the highest expression level of all three CAD isozymes in Carthamus tinctorius Carthamus tinctorius

Inhibitors

Inhibitors Comment Organism Structure
coniferyl aldehyde competitve, a stronger inhibitor than sinapyl aldehyde; competitve, a stronger inhibitor than sinapyl aldehyde; competitve, a stronger inhibitor than sinapyl aldehyde Carthamus tinctorius
sinapyl aldehyde competitve, a weaker inhibitor than coniferyl aldehyde; competitve, a weaker inhibitor than coniferyl aldehyde; competitve, a weaker inhibitor than coniferyl aldehyde Carthamus tinctorius

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0058
-
sinapyl aldehyde pH 6.5-7.5, 26°C, recombinant enzyme Carthamus tinctorius
0.0068
-
coniferyl aldehyde pH 6.5-7.5, 26°C, recombinant enzyme Carthamus tinctorius
0.024
-
coniferyl aldehyde pH 6.5-7.5, 30°C, recombinant enzyme Carthamus tinctorius
0.026
-
sinapyl aldehyde pH 6.5-7.5, 30°C, recombinant enzyme Carthamus tinctorius
0.032
-
coniferyl aldehyde pH 6.5-7.5, 30°C, recombinant enzyme Carthamus tinctorius
0.055
-
sinapyl aldehyde pH 6.5-7.5, 30°C, recombinant enzyme Carthamus tinctorius

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ required, Zn1 binding sequence is located at amino acid residues 68-82, and Zn2 binding sequence is located at amino acid residues 88-113 Carthamus tinctorius
Zn2+ required, Zn1 binding sequence is located at amino acid residues 69-83, and Zn2 binding sequence is located at amino acid residues 89-114 Carthamus tinctorius
Zn2+ required, Zn1 binding sequence is located at amino acid residues 71-85, and Zn2 binding sequence is located at amino acid residues 91-116 Carthamus tinctorius

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cinnamaldehyde + NADPH + H+ Carthamus tinctorius
-
cinnamyl alcohol + NADP+
-
r
coniferyl aldehyde + NADPH + H+ Carthamus tinctorius
-
coniferyl alcohol + NADP+
-
r
sinapyl aldehyde + NADPH + H+ Carthamus tinctorius
-
sinapyl alcohol + NADP+
-
r

Organism

Organism UniProt Comment Textmining
Carthamus tinctorius U3TDQ6 CAD3
-
Carthamus tinctorius U3TH11 CAD2
-
Carthamus tinctorius U3TI46 CAD1
-

Source Tissue

Source Tissue Comment Organism Textmining
seed quantitative realtime PCR enzyme expression analysis during seed development, overview. CAD2 shows the highest expression level of all three CAD isozymes in Carthamus tinctorius Carthamus tinctorius
-
stem
-
Carthamus tinctorius
-
stem low expression of CAD3 in stem Carthamus tinctorius
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cinnamaldehyde + NADPH + H+
-
Carthamus tinctorius cinnamyl alcohol + NADP+
-
r
coniferyl aldehyde + NADPH + H+
-
Carthamus tinctorius coniferyl alcohol + NADP+
-
r
sinapyl aldehyde + NADPH + H+
-
Carthamus tinctorius sinapyl alcohol + NADP+
-
r

Synonyms

Synonyms Comment Organism
CAD
-
Carthamus tinctorius
cinnamyl alcohol dehydrogenase
-
Carthamus tinctorius
CtCAD1
-
Carthamus tinctorius
CtCAD2
-
Carthamus tinctorius
CtCAD3
-
Carthamus tinctorius

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
26
-
assay at Carthamus tinctorius
30
-
assay at Carthamus tinctorius

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00098
-
coniferyl aldehyde pH 6.5-7.5, 30°C, recombinant enzyme Carthamus tinctorius
0.00135
-
sinapyl aldehyde pH 6.5-7.5, 30°C, recombinant enzyme Carthamus tinctorius
0.0053
-
sinapyl aldehyde pH 6.5-7.5, 26°C, recombinant enzyme Carthamus tinctorius
0.0121
-
coniferyl aldehyde pH 6.5-7.5, 26°C, recombinant enzyme Carthamus tinctorius
0.032
-
coniferyl aldehyde pH 6.5-7.5, 30°C, recombinant enzyme Carthamus tinctorius
0.041
-
sinapyl aldehyde pH 6.5-7.5, 30°C, recombinant enzyme Carthamus tinctorius

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7.5 assay at Carthamus tinctorius

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Carthamus tinctorius
NADPH the NADPH binding sequence is located at amino acid residues 188-193 Carthamus tinctorius
NADPH the NADPH binding sequence is located at amino acid residues 189-194 Carthamus tinctorius
NADPH the NADPH binding sequence is located at amino acid residues 191-196 Carthamus tinctorius

General Information

General Information Comment Organism
additional information enzyme structure modelling and molecular docking, substrate binding pocket structure, overview Carthamus tinctorius

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0245
-
sinapyl aldehyde pH 6.5-7.5, 30°C, recombinant enzyme Carthamus tinctorius
0.0306
-
coniferyl aldehyde pH 6.5-7.5, 30°C, recombinant enzyme Carthamus tinctorius
1.32
-
coniferyl aldehyde pH 6.5-7.5, 30°C, recombinant enzyme Carthamus tinctorius
1.58
-
sinapyl aldehyde pH 6.5-7.5, 30°C, recombinant enzyme Carthamus tinctorius
5.39
-
sinapyl aldehyde pH 6.5-7.5, 26°C, recombinant enzyme Carthamus tinctorius
10.62
-
coniferyl aldehyde pH 6.5-7.5, 26°C, recombinant enzyme Carthamus tinctorius