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Literature summary for 1.1.1.195 extracted from

  • Sun, Y.; Wu, Y.; Zhao, Y.; Han, X.; Lou, H.; Cheng, A.
    Molecular cloning and biochemical characterization of two cinnamyl alcohol dehydrogenases from a liverwort Plagiochasma appendiculatum (2013), Plant Physiol. Biochem., 70, 133-141.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene PaCAD1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Plagiochasma appendiculatum
gene PaCAD2, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Plagiochasma appendiculatum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03115
-
coniferaldehyde isoform CAD2, at pH 6.0 and 25°C Plagiochasma appendiculatum
0.0382
-
4-coumarylaldehyde isoform CAD2, at pH 6.0 and 25°C Plagiochasma appendiculatum
0.06095
-
sinapaldehyde isoform CAD2, at pH 6.0 and 25°C Plagiochasma appendiculatum
0.06592
-
caffeylaldehyde isoform CAD2, at pH 6.0 and 25°C Plagiochasma appendiculatum
0.08554
-
coniferaldehyde isoform CAD1, at pH 6.0 and 25°C Plagiochasma appendiculatum
0.09157
-
sinapaldehyde isoform CAD1, at pH 6.0 and 25°C Plagiochasma appendiculatum
0.09318
-
5-hydroxyconiferylaldehyde isoform CAD1, at pH 6.0 and 25°C Plagiochasma appendiculatum
0.09463
-
caffeylaldehyde isoform CAD1, at pH 6.0 and 25°C Plagiochasma appendiculatum
0.1122
-
4-coumarylaldehyde isoform CAD1, at pH 6.0 and 25°C Plagiochasma appendiculatum
0.152
-
5-hydroxyconiferylaldehyde isoform CAD2, at pH 6.0 and 25°C Plagiochasma appendiculatum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35700
-
-
Plagiochasma appendiculatum
36900
-
-
Plagiochasma appendiculatum
55000 60000 gel filtration Plagiochasma appendiculatum

Organism

Organism UniProt Comment Textmining
Plagiochasma appendiculatum
-
-
-
Plagiochasma appendiculatum T1SEU9
-
-
Plagiochasma appendiculatum T1SEV0
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chrmatography Plagiochasma appendiculatum
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Plagiochasma appendiculatum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-coumarylaldehyde + NADPH + H+ high catalytic efficiency Plagiochasma appendiculatum 4-coumaryl alcohol + NADP+
-
?
5-hydroxyconiferyl aldehyde + NADP+ very low activity Plagiochasma appendiculatum 5-hydroxyconiferyl alcohol + NADPH + H+
-
?
5-hydroxyconiferylaldehyde + NADP+ very low activity Plagiochasma appendiculatum 5-hydroxyconiferyl alcohol + NADPH + H+
-
?
5-hydroxyconiferylaldehyde + NADPH + H+ lowest catalytic efficiency Plagiochasma appendiculatum 5-hydroxyconiferyl alcohol + NADP+
-
?
caffeylaldehyde + NADP+ low activity Plagiochasma appendiculatum caffeyl alcohol + NADPH + H+
-
?
caffeylaldehyde + NADPH + H+
-
Plagiochasma appendiculatum caffeyl alcohol + NADP+
-
?
coniferaldehyde + NADPH + H+ favourite substrate Plagiochasma appendiculatum coniferyl alcohol + NADP+
-
?
coniferyl aldehyde + NADPH + H+ best substrate Plagiochasma appendiculatum coniferyl alcohol + NADP+
-
?
p-coumaryl aldehyde + NADPH + H+
-
Plagiochasma appendiculatum p-coumaryl alcohol + NADP+
-
?
p-coumarylaldehyde + NADPH + H+
-
Plagiochasma appendiculatum p-coumaryl alcohol + NADP+
-
?
sinapaldehyde + NADPH + H+
-
Plagiochasma appendiculatum sinapyl alcohol + NADP+
-
?
sinapyl aldehyde + NADPH + H+ low activity Plagiochasma appendiculatum sinapyl alcohol + NADP+
-
?

Subunits

Subunits Comment Organism
? x * 35700, about, CAD1, sequence calculation Plagiochasma appendiculatum
? x * 36900, about, CAD2, sequence calculation Plagiochasma appendiculatum
homodimer 2 * 35700, isoform CAD1, calculated from amino acid sequence Plagiochasma appendiculatum
homodimer 2 * 36900, isoform CAD2, calculated from amino acid sequence Plagiochasma appendiculatum

Synonyms

Synonyms Comment Organism
CAD1
-
Plagiochasma appendiculatum
CAD1 isoform Plagiochasma appendiculatum
CAD2
-
Plagiochasma appendiculatum
CAD2 isoform Plagiochasma appendiculatum
cinnamyl alcohol dehydrogenase
-
Plagiochasma appendiculatum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
-
Plagiochasma appendiculatum
25
-
isoform CAD1 Plagiochasma appendiculatum
30
-
-
Plagiochasma appendiculatum
30
-
isoform CAD2 Plagiochasma appendiculatum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.005
-
5-hydroxyconiferylaldehyde isoform CAD1, at pH 6.0 and 25°C Plagiochasma appendiculatum
0.005
-
sinapaldehyde isoform CAD2, at pH 6.0 and 25°C Plagiochasma appendiculatum
0.006
-
sinapaldehyde isoform CAD1, at pH 6.0 and 25°C Plagiochasma appendiculatum
0.007
-
5-hydroxyconiferylaldehyde isoform CAD2, at pH 6.0 and 25°C Plagiochasma appendiculatum
0.062
-
caffeylaldehyde isoform CAD2, at pH 6.0 and 25°C Plagiochasma appendiculatum
0.072
-
caffeylaldehyde isoform CAD1, at pH 6.0 and 25°C Plagiochasma appendiculatum
0.072
-
coniferaldehyde isoform CAD2, at pH 6.0 and 25°C Plagiochasma appendiculatum
0.085
-
coniferaldehyde isoform CAD1, at pH 6.0 and 25°C Plagiochasma appendiculatum
0.273
-
4-coumarylaldehyde isoform CAD2, at pH 6.0 and 25°C Plagiochasma appendiculatum
0.367
-
4-coumarylaldehyde isoform CAD1, at pH 6.0 and 25°C Plagiochasma appendiculatum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
aldehyde reduction Plagiochasma appendiculatum
5.5
-
isoform CAD2 Plagiochasma appendiculatum
6
-
aldehyde reduction Plagiochasma appendiculatum
6
-
isoform CAD1 Plagiochasma appendiculatum

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Plagiochasma appendiculatum
NADPH
-
Plagiochasma appendiculatum

Expression

Organism Comment Expression
Plagiochasma appendiculatum the expression of isoforms CAD1 and CAD2 is induced by methyl jasmonic acid treatment up
Plagiochasma appendiculatum the expression of PaCAD1 is induced by methyl jasmonic acid (MeJA) treatment by about 2fold up
Plagiochasma appendiculatum the expression of PaCAD2 is induced by methyl jasmonic acid (MeJA) treatment by up to 7fold up

General Information

General Information Comment Organism
physiological function the enzyme plays a role in lignin biosynthesis and the defencedefence of abiotic stresses Plagiochasma appendiculatum
physiological function the two PaCADs, PaCAD1 and PaCAD2, play twin roles in lignin biosynthesis and the defence of abiotic stress in Plagiochasma appendiculatum Plagiochasma appendiculatum
physiological function the two PaCADs, PaCAD1 and PaCAD2, play twin roles in lignin biosynthesis and the defencedefence of abiotic stress in Plagiochasma appendiculatum Plagiochasma appendiculatum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0448
-
5-hydroxyconiferylaldehyde isoform CAD2, at pH 6.0 and 25°C Plagiochasma appendiculatum
0.054
-
5-hydroxyconiferylaldehyde isoform CAD1, at pH 6.0 and 25°C Plagiochasma appendiculatum
0.063
-
sinapaldehyde isoform CAD1, at pH 6.0 and 25°C Plagiochasma appendiculatum
0.075
-
sinapaldehyde isoform CAD2, at pH 6.0 and 25°C Plagiochasma appendiculatum
0.766
-
caffeylaldehyde isoform CAD1, at pH 6.0 and 25°C Plagiochasma appendiculatum
0.935
-
caffeylaldehyde isoform CAD2, at pH 6.0 and 25°C Plagiochasma appendiculatum
0.993
-
coniferaldehyde isoform CAD1, at pH 6.0 and 25°C Plagiochasma appendiculatum
2.322
-
coniferaldehyde isoform CAD2, at pH 6.0 and 25°C Plagiochasma appendiculatum
3.273
-
4-coumarylaldehyde isoform CAD1, at pH 6.0 and 25°C Plagiochasma appendiculatum
7.136
-
4-coumarylaldehyde isoform CAD2, at pH 6.0 and 25°C Plagiochasma appendiculatum