Application | Comment | Organism |
---|---|---|
synthesis | synthesis of cinnamyl alcohol from cinnamaldehyde using the isolated enzyme or by expression of enzyme in Escherichia coli. The reduction of cinnamaldehyde by the isolated enzyme occurrs in 3 h at 50°C with 97% conversion, and yields high purity cinnamyl alcohol with a yield of 88% and a productivity of 50 g/g enzyme. The reduction of 12.5 g/l cinnamaldehyde by whole cells in 6 h, at 37 °C and no requirement of external cofactor occurrs with 97% conversion, 82% yield of 98% pure alcohol and a productivity of 34 mg/g wet cell weight | Geobacillus stearothermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.016 | - |
NADH | pH 7.5, 50°C | Geobacillus stearothermophilus | |
0.03 | - |
cinnamaldehyde | pH 7.5, 50°C | Geobacillus stearothermophilus | |
0.11 | - |
cinnamyl alcohol | pH 8.8, 50°C | Geobacillus stearothermophilus | |
0.45 | - |
NAD+ | pH 8.8, 50°C | Geobacillus stearothermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cinnamaldehyde + NADH + H+ | Geobacillus stearothermophilus | - |
cinnamyl alcohol + NAD+ | - |
r | |
cinnamyl alcohol + NAD+ | Geobacillus stearothermophilus | - |
cinnamaldehyde + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cinnamaldehyde + NADH + H+ | - |
Geobacillus stearothermophilus | cinnamyl alcohol + NAD+ | - |
r | |
cinnamyl alcohol + NAD+ | - |
Geobacillus stearothermophilus | cinnamaldehyde + NADH + H+ | - |
r |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
33 | - |
NAD+ | pH 8.8, 50°C | Geobacillus stearothermophilus | |
43 | - |
NADH | pH 7.5, 50°C | Geobacillus stearothermophilus | |
43 | - |
cinnamyl alcohol | pH 8.8, 50°C | Geobacillus stearothermophilus | |
55 | - |
cinnamaldehyde | pH 7.5, 50°C | Geobacillus stearothermophilus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
73 | - |
NAD+ | pH 8.8, 50°C | Geobacillus stearothermophilus | |
391 | - |
cinnamyl alcohol | pH 8.8, 50°C | Geobacillus stearothermophilus | |
1835 | - |
cinnamaldehyde | pH 7.5, 50°C | Geobacillus stearothermophilus | |
2687 | - |
NADH | pH 7.5, 50°C | Geobacillus stearothermophilus |