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Literature summary for 1.1.1.195 extracted from
- Gold-hull-and-internode2-gene encodes a primarily multifunctional cinnamyl-alcohol dehydrogenase in rice (2006), Plant Physiol., 140, 972-983.
Application
| Application | Comment | Organism |
|---|---|---|
| agriculture | gene GH2 can be useful in engineering of rice, to optimize the important crop plant | Oryza sativa |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene Gold-hull-and-internode2, i.e. gene GH2, DNA and amino acid sequence determination and analysis, map-based cloning approach, phylogenetic and expression pattern analysis, expression of GH2 in Escherichia coli | Oryza sativa |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | the GH2-mutant cells are enzyme-inactive and lignin-deficient | Oryza sativa |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics | Oryza sativa | |
| 0.0044 | - |
coniferyl aldehyde | pH 8.8, 30°C, recombinant enzyme encoded by GH2 | Oryza sativa |  |
| 0.0208 | - |
sinapyl aldehyde | pH 8.8, 30°C, recombinant enzyme encoded by GH2 | Oryza sativa | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryza sativa | - |
gene Gold-hull-and-internode2, i.e. gene GH2 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant GH2-encoded CAD from Escherichia coli | Oryza sativa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| husk | - |
Oryza sativa | - |
| internode | - |
Oryza sativa | - |
| panicle | - |
Oryza sativa | - |
| root | - |
Oryza sativa | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0014 | - |
substrate coniferyl aldehyde, recombinant enzyme encoded by GH2 | Oryza sativa |
| 0.0036 | - |
substrate sinapyl aldehyde, recombinant enzyme encoded by GH2 | Oryza sativa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| coniferyl aldehyde + NADPH | recombinant enzyme encoded by gene GH2 | Oryza sativa | coniferyl alcohol + NADP+ | - |
r | |
| additional information | gene GH2 encodes a multifunctional enzyme | Oryza sativa | ? | - |
? | |
| sinapyl aldehyde + NADPH + H+ | recombinant enzyme encoded by gene GH2 | Oryza sativa | sinapyl alcohol + NADP+ | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CAD | - |
Oryza sativa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Oryza sativa |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.3 | - |
coniferyl aldehyde | pH 8.8, 30°C, recombinant enzyme encoded by GH2 | Oryza sativa | |
| 3.4 | - |
sinapyl aldehyde | pH 8.8, 30°C, recombinant enzyme encoded by GH2 | Oryza sativa | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.8 | - |
assay at | Oryza sativa |
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Release 2023.1 (January 2023)
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