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Literature summary for 1.1.1.195 extracted from
- Metabolite profiling reveals a role for atypical cinnamyl alcohol dehydrogenase CAD1 in the synthesis of coniferyl alcohol in tobacco xylem (2005), Plant Mol. Biol., 59, 753-769.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene CAD1-1, DNA and amino acid sequence determination and analysis, expression analysis, recombinant expression as GST-fusion enzyme in Escherichia coli strain BL21 | Nicotiana tabacum |
| gene CAD1-7, DNA and amino acid sequence determination and analysis, expression analysis, recombinant expression as GST-fusion enzyme in Escherichia coli strain BL21 | Nicotiana tabacum |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of transgenic tobacco plants with reduced expression of CAD1 by RNAi displaying normal growth and development and slight effects on lignin production, but the xylem shows an altered content in phenolic lignin forming compounds, metanolic profiling | Nicotiana tabacum |
| additional information | construction of transgenic tobacco plants with reduced expression of CAD1 by RNAi, transformation using the Agrobacterium tumefaciens system, the transgenic plants display normal growth and development and slight effects on lignin production, but the xylem shows an altered content in lignin forming compounds, metanolic profiling by gas and liquid chromatography mass spectrometry | Nicotiana tabacum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.031 | - |
coniferyl aldehyde | pH 8.8, 30°C, recombinant CAD1-7 | Nicotiana tabacum |  |
| 0.053 | - |
coniferyl aldehyde | pH 10.5, 30°C, recombinant CAD1-1 | Nicotiana tabacum | |
| 0.247 | - |
coniferyl alcohol | pH 6.25, 30°C, recombinant CAD1-1 | Nicotiana tabacum | |
| 0.325 | - |
coniferyl alcohol | pH 6.25, 30°C, recombinant CAD1-7 | Nicotiana tabacum | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| coniferyl aldehyde + NADPH | Nicotiana tabacum | CAD1 and CAD2 are involved in biosynthesis of coniferyl alcohol in Nicotiana tabacum | coniferyl alcohol + NADP+ | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Nicotiana tabacum | Q5D4P8 | gene CAD1-7; cv. Samsun NN | - |
| Nicotiana tabacum | Q5D4P9 | CAD1-1; cv. Samsun NN | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant GST-fusion enzyme from Escherichia coli strain BL21 by glutathione affinity chromatography | Nicotiana tabacum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| coniferyl aldehyde + NADPH | - |
Nicotiana tabacum | coniferyl alcohol + NADP+ | - |
r | |
| coniferyl aldehyde + NADPH | CAD1 and CAD2 are involved in biosynthesis of coniferyl alcohol in Nicotiana tabacum | Nicotiana tabacum | coniferyl alcohol + NADP+ | - |
r | |
| additional information | mo activity with sinapyl aldehyde | Nicotiana tabacum | ? | - |
? | |
| additional information | no activity with sinapaldehyde | Nicotiana tabacum | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CAD1 | - |
Nicotiana tabacum |
| cinnamyl alcohol dehydrogenase 1 | - |
Nicotiana tabacum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Nicotiana tabacum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.17 | - |
coniferyl aldehyde | pH 10.5, 30°C, recombinant CAD1-1 | Nicotiana tabacum | |
| 0.24 | - |
coniferyl aldehyde | pH 8.8, 30°C, recombinant CAD1-7 | Nicotiana tabacum | |
| 0.43 | - |
coniferyl alcohol | pH 6.25, 30°C, recombinant CAD1-1 | Nicotiana tabacum | |
| 0.99 | - |
coniferyl alcohol | pH 6.25, 30°C, recombinant CAD1-7 | Nicotiana tabacum | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.25 | - |
assay at, forward reaction | Nicotiana tabacum |
| 8.8 | - |
reverse reaction | Nicotiana tabacum |
| 10.5 | - |
reverse reaction | Nicotiana tabacum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Nicotiana tabacum | |
| NADPH | - |
Nicotiana tabacum | |
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Release 2023.1 (January 2023)
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