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Literature summary for 1.1.1.195 extracted from

  • Youn, B.; Camacho, R.; Moinuddin, S.G.; Lee, C.; Davin, L.B.; Lewis, N.G.; Kang, C.
    Crystal structures and catalytic mechanism of the Arabidopsis cinnamyl alcohol dehydrogenases AtCAD5 and AtCAD4 (2006), Org. Biomol. Chem., 4, 1687-1697.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
isozyme CAD4, expression in Escherichia coli Arabidopsis thaliana
isozyme CAD5, expression in Escherichia coli Arabidopsis thaliana

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant isozyme CAD5, 4-10 mg/ml apo-protein in 20 mM Tris-HCl, pH 8.0, 1 mM EDTA, and 1 mM DTT, hanging drop vapour diffusion method, at 4°C or at 20°C, 2fold dilution with reservoir solution containing 20% w/v PEG 3350, and 0.2 M trilithium citrate tetrahydrate, pH 8.1 3 days, X-ray diffraction structure determination an analysis at 2.0-2.6 A resolution Arabidopsis thaliana

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ ligand binding structure, Zn2+ is bound in a tetrahedral coordination involving Glu70 Arabidopsis thaliana
Zn2+ zinc-type enzyme, ligand binding structure, Zn2+ is bound in a tetrahedral coordination involving Glu70 Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cinnamyl aldehyde + NADPH Arabidopsis thaliana
-
cinnamyl alcohol + NADP+
-
r
coniferyl aldehyde + NADPH Arabidopsis thaliana
-
coniferyl alcohol + NADP+
-
r

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana O49482 isozyme CAD5; isozyme CAD5
-
Arabidopsis thaliana P48523 isozyme CAD4; isozyme CAD4
-

Purification (Commentary)

Purification (Comment) Organism
recombinant isozyme CAD4 from Escherichia coli Arabidopsis thaliana
recombinant isozyme CAD5 from Escherichia coli Arabidopsis thaliana

Reaction

Reaction Comment Organism Reaction ID
cinnamyl alcohol + NADP+ = cinnamaldehyde + NADPH + H+ catalytic reaction mechanism, substrate and cofactor binding structure Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-hydroxyconiferyl aldehyde + NADPH
-
Arabidopsis thaliana 5-hydroxyconiferyl alcohol + NADP+
-
r
caffeyl aldehyde + NADPH
-
Arabidopsis thaliana caffeyl alcohol + NADP+
-
r
cinnamyl aldehyde + NADPH
-
Arabidopsis thaliana cinnamyl alcohol + NADP+
-
r
coniferyl aldehyde + NADPH
-
Arabidopsis thaliana coniferyl alcohol + NADP+
-
r
coumaryl aldehyde + NADPH + H+
-
Arabidopsis thaliana coumaryl alcohol + NADP+
-
r
additional information substrate specificity of isozyme CAD4 Arabidopsis thaliana ?
-
?
additional information substrate specificity of isozyme CAD5 Arabidopsis thaliana ?
-
?
sinapyl aldehyde + NADPH + H+
-
Arabidopsis thaliana sinapyl alcohol + NADP+
-
r

Subunits

Subunits Comment Organism
dimer crystal structure Arabidopsis thaliana
More structure analysis of isozyme CAD4 by dynamic light scattering Arabidopsis thaliana
More structure analysis of isozyme CAD5 by dynamic light scattering Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
CAD
-
Arabidopsis thaliana
cinnamyl alcohol dehydrogenase
-
Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
NADP+ cofactor binding site structure determination and analysis Arabidopsis thaliana
NADPH cofactor binding site structure determination and analysis Arabidopsis thaliana