Cloned (Comment) | Organism |
---|---|
isozyme CAD4, expression in Escherichia coli | Arabidopsis thaliana |
isozyme CAD5, expression in Escherichia coli | Arabidopsis thaliana |
Crystallization (Comment) | Organism |
---|---|
purified recombinant isozyme CAD5, 4-10 mg/ml apo-protein in 20 mM Tris-HCl, pH 8.0, 1 mM EDTA, and 1 mM DTT, hanging drop vapour diffusion method, at 4°C or at 20°C, 2fold dilution with reservoir solution containing 20% w/v PEG 3350, and 0.2 M trilithium citrate tetrahydrate, pH 8.1 3 days, X-ray diffraction structure determination an analysis at 2.0-2.6 A resolution | Arabidopsis thaliana |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | ligand binding structure, Zn2+ is bound in a tetrahedral coordination involving Glu70 | Arabidopsis thaliana | |
Zn2+ | zinc-type enzyme, ligand binding structure, Zn2+ is bound in a tetrahedral coordination involving Glu70 | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cinnamyl aldehyde + NADPH | Arabidopsis thaliana | - |
cinnamyl alcohol + NADP+ | - |
r | |
coniferyl aldehyde + NADPH | Arabidopsis thaliana | - |
coniferyl alcohol + NADP+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | O49482 | isozyme CAD5; isozyme CAD5 | - |
Arabidopsis thaliana | P48523 | isozyme CAD4; isozyme CAD4 | - |
Purification (Comment) | Organism |
---|---|
recombinant isozyme CAD4 from Escherichia coli | Arabidopsis thaliana |
recombinant isozyme CAD5 from Escherichia coli | Arabidopsis thaliana |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
cinnamyl alcohol + NADP+ = cinnamaldehyde + NADPH + H+ | catalytic reaction mechanism, substrate and cofactor binding structure | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-hydroxyconiferyl aldehyde + NADPH | - |
Arabidopsis thaliana | 5-hydroxyconiferyl alcohol + NADP+ | - |
r | |
caffeyl aldehyde + NADPH | - |
Arabidopsis thaliana | caffeyl alcohol + NADP+ | - |
r | |
cinnamyl aldehyde + NADPH | - |
Arabidopsis thaliana | cinnamyl alcohol + NADP+ | - |
r | |
coniferyl aldehyde + NADPH | - |
Arabidopsis thaliana | coniferyl alcohol + NADP+ | - |
r | |
coumaryl aldehyde + NADPH + H+ | - |
Arabidopsis thaliana | coumaryl alcohol + NADP+ | - |
r | |
additional information | substrate specificity of isozyme CAD4 | Arabidopsis thaliana | ? | - |
? | |
additional information | substrate specificity of isozyme CAD5 | Arabidopsis thaliana | ? | - |
? | |
sinapyl aldehyde + NADPH + H+ | - |
Arabidopsis thaliana | sinapyl alcohol + NADP+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | crystal structure | Arabidopsis thaliana |
More | structure analysis of isozyme CAD4 by dynamic light scattering | Arabidopsis thaliana |
More | structure analysis of isozyme CAD5 by dynamic light scattering | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
CAD | - |
Arabidopsis thaliana |
cinnamyl alcohol dehydrogenase | - |
Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | cofactor binding site structure determination and analysis | Arabidopsis thaliana | |
NADPH | cofactor binding site structure determination and analysis | Arabidopsis thaliana |