Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.195 extracted from

  • Valencia, E.; Larroy, C.; Ochoa, W.F.; Pares, X.; Fita, I.; Biosca, J.A.
    Apo and Holo structures of an NADP(H)-dependent cinnamyl alcohol dehydrogenase from Saccharomyces cerevisiae (2004), J. Mol. Biol., 341, 1049-1062.
    View publication on PubMed
Search for more literature for 1.1.1.195

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in strain BL9 Saccharomyces cerevisiae

Crystallization (Commentary)

Crystallization (Comment) Organism
10 mg/ml purified recombinant enzyme, hanging drop vapour diffusion method, 0.001 ml of both protein and reservoir solution, the latter containing 1. 1.6 M ammonium sulfate, 0.1 M HEPES, pH 7.0, or 2.30% w/v PEG 4000, 0.1 M Tris-HCl, pH 8.5, room temperature, about 1 week, trigonal or monoclinic crystals, X-ray diffraction structure determination and analysis at 3.1 A resolution Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ 2 zinc atoms per subunit Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
 ScAdh6p
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from strain BL9 by DEAE ion exchange, Red-Sepharose, and gel filtration chromatography Saccharomyces cerevisiae

Reaction

Reaction Comment Organism Reaction ID
cinnamyl alcohol + NADP+ = cinnamaldehyde + NADPH + H+ active site structure Saccharomyces cerevisiae
a

Subunits

Subunits Comment Organism
More analysis of the apo and holo enzyme structures Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
CAD
-
 Saccharomyces cerevisiae
cinnamyl alcohol dehydrogenase
-
 Saccharomyces cerevisiae
ScAdh6p
-
 Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
NADPH dependent for, binding and specificity mechanism, interaction through Ser210, Arg211, and Lys215 and the terminal phosphate group Saccharomyces cerevisiae