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Literature summary for 1.1.1.195 extracted from

  • Wyrambik, D.; Grisebach, H.
    Purification and properties of isoenzymes of cinnamyl-alcohol dehydrogenase from soybean-cell-suspension cultures (1975), Eur. J. Biochem., 59, 9-15.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0017
-
coniferaldehyde
-
Glycine max
0.0025
-
cinnamaldehyde
-
Glycine max
0.0038
-
NADP+
-
Glycine max
0.0043
-
sinapaldehyde
-
Glycine max
0.0069
-
3,4-dimethoxycinnamaldehyde
-
Glycine max
0.0072
-
NADPH
-
Glycine max
0.0091
-
p-coumaraldehyde
-
Glycine max
0.011
-
coniferyl alcohol
-
Glycine max
0.022
-
3,4-dimethoxycinnamyl alcohol
-
Glycine max
0.026
-
cinnamyl alcohol
-
Glycine max
0.05
-
sinapyl alcohol
-
Glycine max
0.057
-
p-coumaryl alcohol
-
Glycine max

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
69000
-
gel filtration Glycine max

Organism

Organism UniProt Comment Textmining
Glycine max
-
var. Mandarin
-

Purification (Commentary)

Purification (Comment) Organism
var. Mandarin Glycine max

Source Tissue

Source Tissue Comment Organism Textmining
cell suspension culture
-
Glycine max
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.86
-
-
Glycine max

Storage Stability

Storage Stability Organism
-20°C, 42 mM 2-mercaptoethanol, 10% ethylene glycol, 5 months Glycine max

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3,4-dimethoxycinnamyl alcohol + NADP+
-
Glycine max 3,4-dimethoxycinnamaldehyde + NADPH
-
r
cinnamyl alcohol + NADP+
-
Glycine max cinnamaldehyde + NADPH
-
r
coniferyl alcohol + NADP+
-
Glycine max coniferyl aldehyde + NADPH + H+
-
r
p-coumaryl alcohol + NADP+
-
Glycine max p-coumaraldehyde + NADPH
-
r
sinapyl alcohol + NADP+
-
Glycine max sinapaldehyde + NADPH
-
r

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
coniferyl aldehyde reduction Glycine max
8.8
-
coniferyl alcohol oxidation Glycine max

pH Range

pH Minimum pH Maximum Comment Organism
6 8 coniferyl aldehyde reduction, pH 6.0: about 70% of activity maximum, pH 8.0: about 30% of activity maximum Glycine max
7.2 9.7 coniferyl alcohol oxidation, pH 7.2: about 40% of activity maximum, pH 9.7: about 35% of activity maximum Glycine max

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Glycine max
NADPH
-
Glycine max