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Literature summary for 1.1.1.192 extracted from

  • Luesch, H.; Hoffmann, D.; Hevel, J.M.; Becker, J.E.; Golakoti, T.; Moore, R.E.
    Biosynthesis of 4-methylproline in cyanobacteria: cloning of nosE and nosF genes and biochemical characterization of the encoded dehydrogenase and reductase activities (2003), J. Org. Chem., 68, 83-91.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene nosE, overexpression in Escherichia coli BL21(DE3) as N-terminally His-tagged enzyme Nostoc sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.24
-
(2S,4S)-5-hydroxyleucine pH 10.0, 42°C, recombinant enzyme Nostoc sp.
1.2
-
(2S)-5-hydroxy-2-aminovaleric acid pH 10.0, 42°C, recombinant enzyme Nostoc sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ dependent on Nostoc sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
x * 35000, about, recombinant enzyme, SDS-PAGE Nostoc sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(2S,4S)-5-hydroxyleucine + NAD+ + H2O Nostoc sp. enzyme reaction is part of the biosynthesis of (2S,4S)-4-methylproline (4S)-5-oxo-L-leucine + NADH
-
r

Organism

Organism UniProt Comment Textmining
Nostoc sp.
-
GSV224, gene nosE
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme by nickel affinity chromatography Nostoc sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2S)-5-hydroxy-2-aminovaleric acid + NAD+ + H2O no activity with the (2R)-isomer Nostoc sp. (2S)-2-amino-5-oxopentanoate + NADH
-
?
(2S,4S)-5-hydroxyleucine + NAD+ + H2O
-
Nostoc sp. (4S)-5-oxo-L-leucine + NADH
-
r
(2S,4S)-5-hydroxyleucine + NAD+ + H2O enzyme reaction is part of the biosynthesis of (2S,4S)-4-methylproline Nostoc sp. (4S)-5-oxo-L-leucine + NADH
-
r
additional information stereospecific abstraction of the 5pro-S hydrogen Nostoc sp. ?
-
?

Subunits

Subunits Comment Organism
? x * 35000, about, recombinant enzyme, SDS-PAGE Nostoc sp.

Synonyms

Synonyms Comment Organism
long-chain dehydrogenase
-
Nostoc sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
42
-
-
Nostoc sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10
-
-
Nostoc sp.

Cofactor

Cofactor Comment Organism Structure
NAD+ specific for the A-side of NAD+, no activity with NADP+ Nostoc sp.