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Literature summary for 1.1.1.19 extracted from

  • Wagschal, K.; Jordan, D.B.; Hart-Cooper, W.M.; Chan, V.J.
    Penicillium camemberti galacturonate reductase C-1 oxidation/reduction of uronic acids and substrate inhibition mitigation by aldonic acids (2020), Int. J. Biol. Macromol., 153, 1090-1098 .
    View publication on PubMed
Search for more literature for 1.1.1.19

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Penicillium camemberti

Inhibitors

Inhibitors Comment Organism Structure
D-galacturonate reaction with galacturonate displays marked substrate inhibition Penicillium camemberti
L-gulonate competitive inhibitors in reaction with D-glucuronate Penicillium camemberti
L-gulono-1,4-lactone competitive inhibitors in reaction with D-glucuronate Penicillium camemberti

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0058
-
 NADPH pH 7.5, 37°C, cosubstrate: D-glucuronate Penicillium camemberti
0.097
-
 D-glucuronate pH 7.5, 37°C, cosubstrate: NADPH, spectrometric assay Penicillium camemberti
0.4
-
 NADH pH 7.5, 37°C, cosubstrate: D-glucuronate Penicillium camemberti
0.57
-
 D-galacturonate pH 7.5, 37°C, cosubstrate: NADPH, spectrometric assay Penicillium camemberti
0.95
-
 D-galacturonate pH 7.5, 37°C, cosubstrate: NADPH, HPLC assay Penicillium camemberti
1.96
-
 D-galacturonate pH 7.5, 37°C, cosubstrate: NADH, spectrometric assay Penicillium camemberti
3.15
-
 D-galacturonate pH 7.5, 37°C, cosubstrate: NADH, HPLC assay Penicillium camemberti
3.34
-
 D-glucuronate pH 7.5, 37°C, cosubstrate: NADPH, HPLC assay Penicillium camemberti
6.3
-
 D-glucuronate pH 7.5, 37°C, cosubstrate: NADH, HPLC assay Penicillium camemberti
6.48
-
 D-glucuronate pH 7.5, 37°C, cosubstrate: NADH, spectrometric assay Penicillium camemberti
7.1
-
 D-glucurono-3,6-lactone pH 7.5, 37°C, cosubstrate: NADPH Penicillium camemberti

Organism

Organism UniProt Comment Textmining
Penicillium camemberti A0A0G4NUS2
-
-
Penicillium camemberti FM 013 A0A0G4NUS2
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-galacturonate + NADH + H+ the enzyme is active on both glucuronic acid and galacturonic acid, with similar substrate specificities (kcat/Km) using the preferred co-substrate NADPH Penicillium camemberti L-galactonate + NAD+
-
 ?
D-galacturonate + NADH + H+ the enzyme is active on both glucuronic acid and galacturonic acid, with similar substrate specificities (kcat/Km) using the preferred co-substrate NADPH Penicillium camemberti FM 013 L-galactonate + NAD+
-
 ?
D-galacturonate + NADPH + H+ the enzyme is active on both glucuronic acid and galacturonic acid, with similar substrate specificities (kcat/Km) using the preferred co-substrate NADPH Penicillium camemberti L-galactonate + NADP+
-
 ?
D-galacturonate + NADPH + H+ the enzyme is active on both glucuronic acid and galacturonic acid, with similar substrate specificities (kcat/Km) using the preferred co-substrate NADPH Penicillium camemberti FM 013 L-galactonate + NADP+
-
 ?
D-glucuronate + NADH + H+ the enzyme is active on both glucuronic acid and galacturonic acid, with similar substrate specificities (kcat/Km) using the preferred co-substrate NADPH Penicillium camemberti D-gulonate + NADH + H+
-
 ?
D-glucuronate + NADH + H+ the enzyme is active on both glucuronic acid and galacturonic acid, with similar substrate specificities (kcat/Km) using the preferred co-substrate NADPH Penicillium camemberti FM 013 D-gulonate + NADH + H+
-
 ?
D-glucuronate + NADPH + H+ the enzyme is active on both glucuronic acid and galacturonic acid, with similar substrate specificities (kcat/Km) using the preferred co-substrate NADPH Penicillium camemberti L-gulonate + NADP+
-
 ?
D-glucuronate + NADPH + H+ the enzyme is active on both glucuronic acid and galacturonic acid, with similar substrate specificities (kcat/Km) using the preferred co-substrate NADPH Penicillium camemberti FM 013 L-gulonate + NADP+
-
 ?
D-glucurono-3,6-lactone + NADPH + H+
-
 Penicillium camemberti L-gulono-1,4-lactone
-
 ?
D-glucurono-3,6-lactone + NADPH + H+
-
 Penicillium camemberti FM 013 L-gulono-1,4-lactone
-
 ?

Synonyms

Synonyms Comment Organism
galacturonate oxidoreductase
-
 Penicillium camemberti
PcGOR
-
 Penicillium camemberti

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 K(T)0.5-value Penicillium camemberti
40
-
 1 h, complete unfolding Penicillium camemberti

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.26
-
 D-glucuronate pH 7.5, 37°C, cosubstrate: NADH, spectrometric assay Penicillium camemberti
2.3
-
 D-glucurono-3,6-lactone pH 7.5, 37°C, cosubstrate: NADPH Penicillium camemberti
4.5
-
 D-galacturonate pH 7.5, 37°C, cosubstrate: NADH, spectrometric assay Penicillium camemberti
7.4
-
 NADH pH 7.5, 37°C, cosubstrate: D-glucuronate Penicillium camemberti
7.6
-
 D-glucuronate pH 7.5, 37°C, cosubstrate: NADH, HPLC assay Penicillium camemberti
8.9
-
 D-galacturonate pH 7.5, 37°C, cosubstrate: NADPH, spectrometric assay Penicillium camemberti
9.6
-
 NADPH pH 7.5, 37°C, cosubstrate: D-glucuronate Penicillium camemberti
11.9
-
 D-glucuronate pH 7.5, 37°C, cosubstrate: NADPH, spectrometric assay Penicillium camemberti
13
-
 D-glucuronate pH 7.5, 37°C, cosubstrate: NADPH, HPLC assay Penicillium camemberti
13.6
-
 D-galacturonate pH 7.5, 37°C, cosubstrate: NADPH, HPLC assay Penicillium camemberti
18.5
-
 D-galacturonate pH 7.5, 37°C, cosubstrate: NADH, HPLC assay Penicillium camemberti

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Penicillium camemberti

Cofactor

Cofactor Comment Organism Structure
NADH clear preference for NADPH as cosubstrate Penicillium camemberti
NADPH clear preference for NADPH as cosubstrate Penicillium camemberti

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.19
-
 D-glucuronate pH 7.5, 37°C, cosubstrate: NADH, spectrometric assay Penicillium camemberti
0.32
-
 D-glucurono-3,6-lactone pH 7.5, 37°C, cosubstrate: NADPH Penicillium camemberti
1.2
-
 D-glucuronate pH 7.5, 37°C, cosubstrate: NADH, HPLC assay Penicillium camemberti
2.3
-
 D-galacturonate pH 7.5, 37°C, cosubstrate: NADH, spectrometric assay Penicillium camemberti
3.89
-
 D-glucuronate pH 7.5, 37°C, cosubstrate: NADPH, HPLC assay Penicillium camemberti
5.87
-
 D-galacturonate pH 7.5, 37°C, cosubstrate: NADH, HPLC assay Penicillium camemberti
12.3
-
 D-glucuronate pH 7.5, 37°C, cosubstrate: NADPH, spectrometric assay Penicillium camemberti
14.3
-
 D-galacturonate pH 7.5, 37°C, cosubstrate: NADPH, HPLC assay Penicillium camemberti
16
-
 D-galacturonate pH 7.5, 37°C, cosubstrate: NADPH, spectrometric assay Penicillium camemberti
19
-
 NADH pH 7.5, 37°C, cosubstrate: D-glucuronate Penicillium camemberti
1655
-
 NADPH pH 7.5, 37°C, cosubstrate: D-glucuronate Penicillium camemberti