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Literature summary for 1.1.1.188 extracted from
- Evaluation of the prostaglandin F synthase activity of human and bovine aldo-keto reductases: AKR1A1s complement AKR1B1s as potent PGF synthases (2013), Prostaglandins Other Lipid Mediat., 106, 124-132.
Application
| Application | Comment | Organism |
|---|---|---|
| drug development | prostaglandin formation is a key component of PGaction and the specific target of non-steroidal anti-inflammatory drugs | Homo sapiens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene AKR1B1, recombinant expression of N-terminally His8-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS | Bos taurus |
| gene AKR1C3, recombinant expression of N-terminally His8-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 9,10-phenanthrenequinone | - |
Bos taurus |  |
| 9,10-phenanthrenequinone | - |
Homo sapiens | |
| methylglyoxal | - |
Bos taurus | |
| methylglyoxal | - |
Homo sapiens | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| prostaglandin H2 + NADP+ | Homo sapiens | - |
prostaglandin F2alpha + NADPH + H+ | - |
? | |
| prostaglandin H2 + NADP+ | Bos taurus | - |
prostaglandin F2alpha + NADPH + H+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | P16116 | - |
- |
| Homo sapiens | P15121 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| uterine endometrium | - |
Homo sapiens | - |
| uterine endometrium | the bovine endometrium produces huge amounts of PGF2alpha | Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.041 | - |
purified recombinant enzyme, pH 7.0, 25°C, in presence of 0.05 mM 9,10-phenanthrenequinone | Bos taurus |
| 0.078 | - |
purified recombinant enzyme, pH 7.0, 25°C, in presence of 0.05 mM 9,10-phenanthrenequinone | Homo sapiens |
| 0.2 | - |
about, purified recombinant enzyme, pH 7.0, 25°C | Homo sapiens |
| 0.41 | - |
about, purified recombinant enzyme, pH 7.0, 25°C | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | PGFS activity of the recombinant proteins is evaluated using an assay based on in situ generation of the precursor of prostaglandin PGH2 biosynthesis. PGF2alpha is measured by ELISA. Comparisons of PGFS activity of recombinant bovine and human endometrial AKRs, overview | Homo sapiens | ? | - |
? | |
| additional information | PGFS activity of the recombinant proteins is evaluated using an assay based on in situ generation of the precursor of prostaglandin PGH2 biosynthesis. PGF2alpha is measured by ELISA. Comparisons of PGFS activity of recombinant bovine and human endometrial AKRs, overview | Bos taurus | ? | - |
? | |
| prostaglandin H2 + NADP+ | - |
Homo sapiens | prostaglandin F2alpha + NADPH + H+ | - |
? | |
| prostaglandin H2 + NADP+ | - |
Bos taurus | prostaglandin F2alpha + NADPH + H+ | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AKR1B1 | - |
Bos taurus |
| AKR1C3 | - |
Homo sapiens |
| More | cf. EC 1.1.1.21 | Homo sapiens |
| PGFS | - |
Homo sapiens |
| PGFS | - |
Bos taurus |
| prostaglandin F synthase | - |
Homo sapiens |
| prostaglandin F synthase | - |
Bos taurus |
| prostaglandin F2alpha synthase | - |
Homo sapiens |
| prostaglandin F2alpha synthase | - |
Bos taurus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Homo sapiens |
| 25 | - |
assay at | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Homo sapiens |
| 7 | - |
assay at | Bos taurus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Homo sapiens | |
| NADP+ | - |
Bos taurus | |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Bos taurus | AKR1B1 expression is induced by interleukin-1beta leading to a higher production of prostaglandin F2alpha | up |
| Homo sapiens | AKR1C3 expression is induced by interleukin-1beta leading to a higher production of prostaglandin F2alpha | up |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | AKR1B1 is able to produce PGF2alpha in the endometrium in addition toAKR1C3. The PGF synthase activity of AKR1B1 proves to be much higher than that of AKR1C3 | Bos taurus |
| metabolism | AKR1C3 is able to produce PGF2alpha in the endometrium in addition to AKR1B1. The PGF synthase activity of AKR1B1 proves to be much higher than that of AKR1C3 | Homo sapiens |
| additional information | comparisons of PGFS activity of recombinant bovine and human endometrial AKRs, overview | Homo sapiens |
| additional information | comparisons of PGFS activity of recombinant bovine and human endometrial AKRs, overview | Bos taurus |
| physiological function | PGF2alpha plays important roles in the regulation of ocular pressure, renal absorption, cardiovascular function, adipocyte differentiation, and female reproductive function. Role of AKR1C3 in the formation of PGF2alpha from PGH2 in the bovine endometrium | Homo sapiens |
| physiological function | PGF2alpha plays important roles in the regulation of ocular pressure, renal absorption, cardiovascular function, adipocyte differentiation, and female reproductive function. Role of bovine AKR1B1, previously known as AKR1B5, in the formation of PGF2alpha from PGH2 in the bovine endometrium | Bos taurus |
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