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Literature summary for 1.1.1.188 extracted from

  • Moriuchi, H.; Koda, N.; Okuda-Ashitaka, E.; Daiyasu, H.; Ogasawara, K.; Toh, H.; Ito, S.; Woodward, D.F.; Watanabe, K.
    Molecular characterization of a novel type of prostamide/prostaglandin F synthase, belonging to the thioredoxin-like superfamily (2008), J. Biol. Chem., 283, 792-801.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
ammonium sulfate activity of the purified recombinant enzyme is stimulated about 4fold by 1.5 M Sus scrofa

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 cells Sus scrofa
wild-type and mutants, plasmid pCold II-PM/PGFS expressed in Escherichia coli BL21 cells Mus musculus

Protein Variants

Protein Variants Comment Organism
C44S retains 63% of the enzyme activity of the wild-type Mus musculus
C44S/C47S shows values below 1% of the enzyme activity of the wild-type Mus musculus
C47S shows values below 1% of the enzyme activity of the wild-type Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0069
-
prostaglandin H2 pH 7.0, 24°C Mus musculus
0.007
-
prostaglandin H2
-
Sus scrofa

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Mus musculus 5737
-
endoplasmic reticulum
-
Mus musculus 5783
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20000
-
immunoprecipitation, Western blot analysis Sus scrofa
23000
-
by zinc staining Mus musculus
23230
-
expressed protein Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-
Sus scrofa A9CQL8
-
-

Purification (Commentary)

Purification (Comment) Organism
on Ni2+ column, 102- and 152fold purification of the prostamide F synthase and PGF synthase activities, respectively, achieved from the swine brain (90 g) with a yield of 1.4 and 2%, respectively. Mutants expressed to homogeneity Sus scrofa
to apparent homogeneity on Ni2+ column Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
adrenal gland
-
Mus musculus
-
brain highest activity Sus scrofa
-
brain high activity Mus musculus
-
cecum low activity Mus musculus
-
eye
-
Sus scrofa
-
eye low activity Mus musculus
-
heart
-
Sus scrofa
-
heart high activity Mus musculus
-
kidney low activity Mus musculus
-
large intestine low activity Mus musculus
-
liver low activity Mus musculus
-
lung
-
Sus scrofa
-
lung low activity Mus musculus
-
additional information low activity in deferent duct and epididymidis Mus musculus
-
muscle low activity Mus musculus
-
ovary high activity Mus musculus
-
small intestine low activity Mus musculus
-
spinal cord
-
Mus musculus
-
spleen low activity Mus musculus
-
stomach low activity Mus musculus
-
testis low activity Mus musculus
-
thymus high activity Mus musculus
-
uterus high activity Mus musculus
-
vesicular gland low activity Mus musculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.062
-
PGF synthase activity of the purified enzyme Sus scrofa
0.69
-
PGF synthase activity of the purified enzyme Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity with prostaglandin D2 and prostaglandin E2 Mus musculus ?
-
?
prostaglandin H2 + NADPH + H+
-
Mus musculus prostaglandin F2alpha + NADP+
-
?
prostaglandin H2 + NADPH + H+
-
Sus scrofa prostaglandin F2alpha + NADP+
-
?

Subunits

Subunits Comment Organism
dimer
-
Sus scrofa
dimer 2 * 23000, wild-type and mutants, SDS-PAGE Mus musculus

Synonyms

Synonyms Comment Organism
prostamide/PGF synthase
-
Mus musculus
prostamide/PGF synthase
-
Sus scrofa
prostamide/prostaglandin F synthase
-
Mus musculus
prostamide/prostaglandin F synthase
-
Sus scrofa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.2467
-
prostaglandin H2
-
Sus scrofa
0.2467
-
prostaglandin H2 pH 7.0, 24°C Mus musculus

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Mus musculus
NADPH
-
Sus scrofa

General Information

General Information Comment Organism
physiological function prostamide/PGF synthase may play an important functional role in the central nervous system Mus musculus
physiological function prostamide/PGF synthase may play an important functional role in the central nervous system. It directly synthesizes prostamide/PGF2alpha from prostamide/PGH2 better than PGF synthase Sus scrofa

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
35.85
-
prostaglandin H2 pH 7.0, 24°C Mus musculus