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Literature summary for 1.1.1.188 extracted from

  • Okano, Y.; Inoue, T.; Kubata, B.K.; Kabututu, Z.; Urade, Y.; Matsumura, H.; Kai, Y.
    Crystallization and preliminary X-ray crystallographic studies of Trypanosoma brucei prostaglandin F2 alpha synthase (2002), J. Biochem., 132, 859-861.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli strain BL21(DE3) as GST-fusion protein Trypanosoma brucei

Crystallization (Commentary)

Crystallization (Comment) Organism
10 mg/ml purified recombinant enzyme from 0.1 M HEPES/NaOH, pH 7.5, 2.0 M ammonium sulfate, 5% w/v PEG 400, and 0.5 M NADPH by hanging drop vapour diffusion method, 20°C, 3 weeks, X-ray diffraction structure determination at room temperature and analysis at 2.6 A resolution Trypanosoma brucei

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Trypanosoma brucei 5829
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
prostaglandin H2 + NADPH + H+ Trypanosoma brucei
-
prostaglandin F2alpha + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Trypanosoma brucei
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli by GSH affinity chromatography, cleavage of the fusion protein glutathione S-tranferase by thrombin, and DEAE ion exchange chromatography Trypanosoma brucei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
9,10-phenanthrenequinone + NADPH
-
Trypanosoma brucei ?
-
?
additional information enzyme does not reduce prostaglandins D2 and E2 Trypanosoma brucei ?
-
?
prostaglandin H2 + NADPH + H+
-
Trypanosoma brucei prostaglandin F2alpha + NADP+
-
?

Synonyms

Synonyms Comment Organism
More enzyme belongs to the AKR aldo-keto reductase superfamily Trypanosoma brucei
PGF2alpha synthase
-
Trypanosoma brucei

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Trypanosoma brucei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Trypanosoma brucei

Cofactor

Cofactor Comment Organism Structure
NADPH dependent on Trypanosoma brucei