Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli strain BL21(DE3) as GST-fusion protein | Trypanosoma brucei |
Crystallization (Comment) | Organism |
---|---|
10 mg/ml purified recombinant enzyme from 0.1 M HEPES/NaOH, pH 7.5, 2.0 M ammonium sulfate, 5% w/v PEG 400, and 0.5 M NADPH by hanging drop vapour diffusion method, 20°C, 3 weeks, X-ray diffraction structure determination at room temperature and analysis at 2.6 A resolution | Trypanosoma brucei |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Trypanosoma brucei | 5829 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
prostaglandin H2 + NADPH + H+ | Trypanosoma brucei | - |
prostaglandin F2alpha + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma brucei | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli by GSH affinity chromatography, cleavage of the fusion protein glutathione S-tranferase by thrombin, and DEAE ion exchange chromatography | Trypanosoma brucei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
9,10-phenanthrenequinone + NADPH | - |
Trypanosoma brucei | ? | - |
? | |
additional information | enzyme does not reduce prostaglandins D2 and E2 | Trypanosoma brucei | ? | - |
? | |
prostaglandin H2 + NADPH + H+ | - |
Trypanosoma brucei | prostaglandin F2alpha + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
More | enzyme belongs to the AKR aldo-keto reductase superfamily | Trypanosoma brucei |
PGF2alpha synthase | - |
Trypanosoma brucei |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Trypanosoma brucei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Trypanosoma brucei |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | dependent on | Trypanosoma brucei |