Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.188 extracted from

  • Komoto, J.; Yamada, T.; Watanabe, K.; Takusagawa, F.
    Crystal structure of human prostaglandin F synthase (AKR1C3) (2004), Biochemistry, 43, 2188-2198.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli HB101 Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
20 mg/ml purified recombinant enzyme in a solution containing 1.4 mM PGD2 or inhibitor rutin, 1.2 mM NADP+ and NADPH, 50 mM MES, pH 6.0, and 25% w/v PEG 8000, hanging drop vapour diffusion method, 2-3 days, thick plate-shaped crystals of enzyme with NADP+ and PGD2 and of enzyme with NADPH and rutin, X-ray diffraction structure determination and analysis at 1.69 A resolution Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
rutin binding structure at the substrate binding site Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P42330
-
-

Reaction

Reaction Comment Organism Reaction ID
(5Z,13E)-(15S)-9alpha,11alpha,15-trihydroxyprosta-5,13-dienoate + NADP+ = (5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate + NADPH + H+ substrate binding and catalytic mechanism, catalytic site residues are Y55 and H117, enzyme also shows 3alpha-hydroxysteroid dehydrogenase type II activity, i.e. 3alpha-HSD Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
lung
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information Y55 and H117 are involved in catalysis, enzyme also shows 3alpha-hydroxysteroid dehydrogenase type II activity Homo sapiens ?
-
?
prostaglandin D2 + NADPH + H+
-
Homo sapiens 9alpha,11beta-prostaglandin F2alpha + NADP+
-
r
prostaglandin H2 + NADPH + H+
-
Homo sapiens prostaglandin F2alpha + NADP+
-
?

Subunits

Subunits Comment Organism
More enzyme has a (alpha/beta)8 barrel structure, from crystal structure determination Homo sapiens

Synonyms

Synonyms Comment Organism
AKR1C3
-
Homo sapiens
More enzyme also shows 3alpha-hydroxysteroid dehydrogenase type II activity, i.e. 3alpha-HSD Homo sapiens
PGFS
-
Homo sapiens
prostaglandin F synthase
-
Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NADP+ binding structure at the active site Homo sapiens
NADPH binding structure at the active site, catalytic structure and mechanism involving the cofactor Homo sapiens