Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli HB101 | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
20 mg/ml purified recombinant enzyme in a solution containing 1.4 mM PGD2 or inhibitor rutin, 1.2 mM NADP+ and NADPH, 50 mM MES, pH 6.0, and 25% w/v PEG 8000, hanging drop vapour diffusion method, 2-3 days, thick plate-shaped crystals of enzyme with NADP+ and PGD2 and of enzyme with NADPH and rutin, X-ray diffraction structure determination and analysis at 1.69 A resolution | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
rutin | binding structure at the substrate binding site | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P42330 | - |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(5Z,13E)-(15S)-9alpha,11alpha,15-trihydroxyprosta-5,13-dienoate + NADP+ = (5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate + NADPH + H+ | substrate binding and catalytic mechanism, catalytic site residues are Y55 and H117, enzyme also shows 3alpha-hydroxysteroid dehydrogenase type II activity, i.e. 3alpha-HSD | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
lung | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Y55 and H117 are involved in catalysis, enzyme also shows 3alpha-hydroxysteroid dehydrogenase type II activity | Homo sapiens | ? | - |
? | |
prostaglandin D2 + NADPH + H+ | - |
Homo sapiens | 9alpha,11beta-prostaglandin F2alpha + NADP+ | - |
r | |
prostaglandin H2 + NADPH + H+ | - |
Homo sapiens | prostaglandin F2alpha + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | enzyme has a (alpha/beta)8 barrel structure, from crystal structure determination | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
AKR1C3 | - |
Homo sapiens |
More | enzyme also shows 3alpha-hydroxysteroid dehydrogenase type II activity, i.e. 3alpha-HSD | Homo sapiens |
PGFS | - |
Homo sapiens |
prostaglandin F synthase | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | binding structure at the active site | Homo sapiens | |
NADPH | binding structure at the active site, catalytic structure and mechanism involving the cofactor | Homo sapiens |