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Literature summary for 1.1.1.185 extracted from
- L-Glycol dehydrogenase from hen muscle (1982), Methods Enzymol., 89, 523-526.
General Stability
| General Stability | Organism |
|---|---|
| easily inactivated in high molarity buffers | Gallus gallus |
| very stable in both water and low molarity buffers of pH 7 at 0-4°C | Gallus gallus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| acetone | pI 7.2 form | Gallus gallus |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Gallus gallus | - |
hen | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 3 enzyme forms: pI 7.2, pI 6.2 and pI 4.8 | Gallus gallus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| an L-glycol + NAD(P)+ = a 2-hydroxycarbonyl compound + NAD(P)H + H+ | glyoxal reduction by enzyme form pI 7.2 follows ordered bi-bi mechanism in which the coenzyme is the first substrate to bind to the enzyme | Gallus gallus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| muscle | - |
Gallus gallus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.4 | - |
- |
Gallus gallus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -18°C, enzyme at any stage of purification | Gallus gallus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| R1-CO-CHOH-R2 + NAD(P)H | - |
Gallus gallus | R1-CHOH-CHOH-R2 + NAD(P)+ | - |
r | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Gallus gallus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 6.6 | - |
Gallus gallus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
very stable in both water and low molarity buffers of pH 7 at 0-4°C | Gallus gallus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Gallus gallus | |
| NADH | - |
Gallus gallus | |
| NADPH | - |
Gallus gallus | |
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Release 2023.1 (January 2023)
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