General Stability | Organism |
---|---|
easily inactivated in high molarity buffers | Gallus gallus |
very stable in both water and low molarity buffers of pH 7 at 0-4°C | Gallus gallus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Gallus gallus | |
0.026 | - |
glyceraldehyde | pI 7.2 form | Gallus gallus | |
0.026 | - |
glyceraldehyde | enzyme form of pI 7.2, at pH 7.0 and 25°C | Gallus gallus | |
0.029 | - |
glyceraldehyde | pI 6.2 form | Gallus gallus | |
0.029 | - |
glyceraldehyde | enzyme form of pI 6.2, at pH 7.0 and 25°C | Gallus gallus | |
0.053 | - |
Glyoxal | pI 6.2 form | Gallus gallus | |
0.053 | - |
Glyoxal | enzyme form of pI 6.2, at pH 7.0 and 25°C | Gallus gallus | |
0.06 | - |
2,3-Pentanedione | pI 7.2 form | Gallus gallus | |
0.097 | - |
2,3-Pentanedione | pI 6.2 form | Gallus gallus | |
0.098 | - |
methylglyoxal | pI 6.2 form | Gallus gallus | |
0.102 | - |
methylglyoxal | pI 7.2 form | Gallus gallus | |
0.109 | - |
diacetyl | pI 7.2 form | Gallus gallus | |
0.38 | - |
diacetyl | pI 6.2 form | Gallus gallus | |
0.411 | - |
Glyoxal | pI 4.8 form | Gallus gallus | |
0.411 | - |
Glyoxal | enzyme form of pI 4.8, at pH 7.0 and 25°C | Gallus gallus | |
0.522 | - |
Glyoxal | pI 7.2 form | Gallus gallus | |
0.522 | - |
Glyoxal | enzyme form of pI 7.2, at pH 7.0 and 25°C | Gallus gallus | |
0.566 | - |
glyceraldehyde | pI 4.8 form | Gallus gallus | |
0.566 | - |
glyceraldehyde | enzyme form of pI 4.8, at pH 7.0 and 25°C | Gallus gallus | |
0.675 | - |
2,3-Pentanedione | pI 4.8 form | Gallus gallus | |
2.013 | - |
acetoin | pI 6.2 form | Gallus gallus | |
2.164 | - |
acetoin | pI 4.8 form | Gallus gallus | |
2.59 | - |
acetoin | pI 7.2 form | Gallus gallus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
gel filtration | Gallus gallus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gallus gallus | - |
hen | - |
Purification (Comment) | Organism |
---|---|
3 enzyme forms: pI 7.2, pI 6.2 and pI 4.8 | Gallus gallus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
muscle | - |
Gallus gallus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Gallus gallus |
Storage Stability | Organism |
---|---|
-18°C, enzyme at any stage of purification | Gallus gallus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,3-pentanedione + NADH | - |
Gallus gallus | L-acetylethylcarbinol + NAD+ | - |
? | |
diacetyl + NADH | - |
Gallus gallus | L-acetoin + NAD+ | - |
? | |
ethyl pyruvate + NAD(P)H | - |
Gallus gallus | ethyl lactate | - |
? | |
glyceraldehyde + NADPH + H+ | - |
Gallus gallus | ? | - |
r | |
glyoxal + NADPH + H+ | - |
Gallus gallus | ? | - |
r | |
methyl pyruvate + NAD(P)H | - |
Gallus gallus | ? | - |
? | |
methylglyoxal + NADH | - |
Gallus gallus | L-lactaldehyde | - |
? | |
additional information | not: acetaldehyde, monoketones, non-vicinal diketones, free ketoacids | Gallus gallus | ? | - |
? | |
R1-CO-CHOH-R2 + NAD(P)H | 2-hydroxycarbonyl compound e.g. glycolaldehyde, glyceraldehyde, acetoin, acetylethylcarbinol, diacetylmethylcarbinol | Gallus gallus | R1-CHOH-CHOH-R2 + NAD(P)+ | - |
r | |
R1-CO-CHOH-R2 + NAD(P)H | much more efficient in dehydrogenase direction | Gallus gallus | R1-CHOH-CHOH-R2 + NAD(P)+ | - |
r | |
R1-CO-CO-R2 + NAD(P)H | methylglyoxal, diacetyl, 2,3-pentanedione | Gallus gallus | R1-CO-CHOH-R2 + NAD(P)+ | - |
? | |
R1-CO-CO-R2 + NAD(P)H | vicinal dicarbonyl: e.g. glyoxal | Gallus gallus | R1-CO-CHOH-R2 + NAD(P)+ | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Gallus gallus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | 6.6 | - |
Gallus gallus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | - |
very stable in both water and low molarity buffers of pH 7 at 0-4°C | Gallus gallus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no activity with alpha-NADH | Gallus gallus | |
NAD+ | - |
Gallus gallus | |
NADH | - |
Gallus gallus | |
NADPH | - |
Gallus gallus |