Application | Comment | Organism |
---|---|---|
synthesis | coexpression with glucose dehydrogenase from Bacillus subtilis for NADPH regeneration in Escherichia coli and optimization of linker peptides used for the fusion expression of carbonyl reductase and glucose dehydrogenase. Up to 297.3 g/L (R)-[3,5-bis(trifluoromethyl)phenyl] ethanol with enantiopurity >99.9% ee is produced via reduction of 1.2 M of substrate with a 96.7% yield and productivity of 29.7 g/(L h) | Lentilactobacillus kefiri |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Lentilactobacillus kefiri |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lentilactobacillus kefiri | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,5-bis(trifluoromethyl) acetophenone + NADPH + H+ | - |
Lentilactobacillus kefiri | (R)-[3,5-bis(trifluoromethyl)phenyl] ethanol + NADP+ | - |
? |