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Literature summary for 1.1.1.184 extracted from

  • Ferguson, D.C.; Cheng, Q.; Blanco, J.G.
    Characterization of the canine snthracycline-metabolizing enzyme carbonyl reductase 1 (cbr1) and the functional isoform cbr1 V218 (2015), Drug Metab. Dispos., 43, 922-927.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.184

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Canis lupus familiaris

Protein Variants

Protein Variants Comment Organism
D218V single nucleotide polymorphism Canis lupus familiaris

Inhibitors

Inhibitors Comment Organism Structure
rutin competitive Canis lupus familiaris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.104
-
 daunorubicin mutant D218V, pH 7.4, 37°C Canis lupus familiaris
0.118
-
 menadione mutant D218V, pH 7.4, 37°C Canis lupus familiaris
0.188
-
 daunorubicin wild-type, pH 7.4, 37°C Canis lupus familiaris
0.527
-
 menadione wild-type, pH 7.4, 37°C Canis lupus familiaris

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32800
-
-
 Canis lupus familiaris

Organism

Organism UniProt Comment Textmining
Canis lupus familiaris J9P7P2
-
-

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Canis lupus familiaris
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
daunorubicin + NADPH + H+
-
 Canis lupus familiaris daunorubicinol + NADP+
-
 ?
menadione + NADPH + H+
-
 Canis lupus familiaris ? + NADP+
-
 ?

Subunits

Subunits Comment Organism
? x * 32800, SDS-PAGE, recombinant His-tagged protein Canis lupus familiaris

Synonyms

Synonyms Comment Organism
CBR1
-
 Canis lupus familiaris
LOC610164 locus name Canis lupus familiaris

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.12
-
 menadione mutant D218V, pH 7.4, 37°C Canis lupus familiaris
1.12
-
 daunorubicin mutant D218V, pH 7.4, 37°C Canis lupus familiaris
3.52
-
 daunorubicin wild-type, pH 7.4, 37°C Canis lupus familiaris
8.48
-
 menadione wild-type, pH 7.4, 37°C Canis lupus familiaris

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0014
-
 mutant D218V, pH 7.4, 37°C Canis lupus familiaris rutin
0.0018
-
 wild-type, pH 7.4, 37°C Canis lupus familiaris rutin