Literature summary for 1.1.1.184 extracted from

Gao, C.; Zhang, L.; Xie, Y.; Hu, C.; Zhang, Y.; Li, L.; Wang, Y.; Ma, C.; Xu, P.
Production of (3S)-acetoin from diacetyl by using stereoselective NADPH-dependent carbonyl reductase and glucose dehydrogenase (2013), Biores. Technol., 137, 111-115.

Search for more literature for 1.1.1.184

Application

Application	Comment	Organism
synthesis	synthesis of (3S)-acetoin in a coupled system consisting of glucose dehydrogenase from Bacillus subtilis 168 and the NADPH-dependent carbonyl reductase. Under the optimal conditions, 12.2 g/l (3S)-acetoin is produced from 14.3 g/l diacetyl in 75 min	Gluconobacter oxydans

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0136	-	diacetyl	pH 7.4, 30°C	Gluconobacter oxydans

Organism

Organism	UniProt	Comment	Textmining
Gluconobacter oxydans	-	-	-
Gluconobacter oxydans DSM 2003	-	-	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
75.9	-	pH 7.4, 30°C	Gluconobacter oxydans

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
diacetyl + NADPH + H+	-	Gluconobacter oxydans	(3S)-acetoin + NADP+	no synthesis of (2S,3S)-2,3-butanediol	?
diacetyl + NADPH + H+	-	Gluconobacter oxydans DSM 2003	(3S)-acetoin + NADP+	no synthesis of (2S,3S)-2,3-butanediol	?

Subunits

Subunits	Comment	Organism
?	x * 3200, SDS-PAGE	Gluconobacter oxydans

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Release 2023.1 (January 2023)