Literature summary for 1.1.1.184 extracted from

Tanaka, N.; Aoki, K.; Ishikura, S.; Nagano, M.; Imamura, Y.; Hara, A.; Nakamura, K.T.
Molecular basis for peroxisomal localization of tetrameric carbonyl reductase (2008), Structure, 16, 388-397.

Search for more literature for 1.1.1.184

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Sus scrofa

Crystallization (Commentary)

Crystallization (Comment)	Organism
C-terminal peroxisomal targeting sequence 1 of each subunit is involved in intersubunit interactions and buried in the interior of the tetrameric enzyme	Sus scrofa

Protein Variants

Protein Variants	Comment	Organism
additional information	mutation of C-terminal peroxisomal targeting sequence SRL to SKL, SHL, SLL, and SL. Mutants SLL and SL are not catalytically active. Mutant SHL is gradually inactivated during incubation at 0°C. Both mutants SHL and SKL are tetramers and show kinetic parameters similar to wild-type	Sus scrofa

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
peroxisome	C-terminal peroxisomal targeting sequence 1 of each subunit is involved in intersubunit interactions and buried in the interior of the tetrameric enzyme	Sus scrofa	5777	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
27000	-	4 * 27000, SDS-PAGE and crystallization data	Sus scrofa
100000	-	gel filtration, wild-type and mutants SHL, SKL	Sus scrofa

Organism

Organism	UniProt	Comment	Textmining
Sus scrofa	Q8WNV7	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
heart	-	Sus scrofa	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1-phenylisatin + NADPH	-	Sus scrofa	? + NADP+	-	?
4-benzoylpyridine + NADPH + H+	-	Sus scrofa	phenyl-4-pyridylmethanol + NADP+	-	?
ethyl benzoylformate + NADPH	-	Sus scrofa	? + NADP+	-	?

Subunits

Subunits	Comment	Organism
tetramer	4 * 27000, SDS-PAGE and crystallization data	Sus scrofa

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Release 2023.1 (January 2023)